Yoon Yong Joo, Jin Woo Park Jin Woo, Lee Eun Jung
Department of Otolaryngology-Head and Neck Surgery, Chonbuk National University Medical School, Research Institute of Clinical Medicine, Chonbuk National University, Jeon-ju, Korea.
Acta Otolaryngol. 2008 Aug;128(8):871-5. doi: 10.1080/00016480701785020.
Human beta-defensin-1 (hBD-1) and human beta-defensin-2 (hBD-2) antimicrobial peptides present in the cerumen, which is composed of exfoliated epithelial keratin and gland secretion, might provide the first line of defense against microbes in external auditory canal (EAC) skin.
Cerumen (earwax) plays a primary role in protecting the EAC skin and tympanic membrane. Even though the protection by antimicrobial peptides present in the skin secretion has been well established, little is known about the intrinsic role of the peptides in the EAC skin and cerumen. The aim of this study was to examine the presence of important antimicrobial peptides, hBD-1 and hBD-2, in the cerumen and EAC skin.
Cerumen was collected from 20 healthy adults, and the EAC skins were obtained from 12 patients who underwent middle ear surgery with canaloplasty. The presence of hBD-1 and hBD-2 was analyzed using immunohistochemistry and Western blotting.
In the immunohistochemical study of the EAC skin, expression of hBD-1 and hBD-2 was observed in both the epithelium and the glands. The presence of hBD-1 and hBD-2 peptides in the cerumen was confirmed by Western blotting.
存在于耵聍中的人β-防御素-1（hBD-1）和人β-防御素-2（hBD-2）抗菌肽，由脱落的上皮角蛋白和腺体分泌物组成，可能对外耳道（EAC）皮肤中的微生物提供第一道防线。
耵聍（耳垢）在保护EAC皮肤和鼓膜方面起主要作用。尽管皮肤分泌物中存在的抗菌肽的保护作用已得到充分证实，但关于这些肽在EAC皮肤和耵聍中的内在作用知之甚少。本研究的目的是检测耵聍和EAC皮肤中重要抗菌肽hBD-1和hBD-2的存在情况。
从20名健康成年人中收集耵聍，并从12例接受中耳手术并行外耳道成形术的患者中获取EAC皮肤。采用免疫组织化学和蛋白质印迹法分析hBD-1和hBD-2的存在情况。
在EAC皮肤的免疫组织化学研究中，在上皮和腺体中均观察到hBD-1和hBD-2的表达。蛋白质印迹法证实了耵聍中存在hBD-1和hBD-2肽。
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