Yoon Y J, Lee E J
Department of Otolaryngology-Head and Neck Surgery, College of Medicine and Research, Institute of Clinical Medicine, Chonbuk National University, Jeonju, South Korea.
J Laryngol Otol. 2011 Nov;125(11):e6. doi: 10.1017/S0022215111002271.
The direct activity of antimicrobial peptides against microbes is thought to be an essential first line of defence in the skin; however, little is known about antimicrobial peptide secretion in the skin of the external auditory canal. Evidence suggests that mast cells contribute to the secretion of antimicrobial peptides. This study aimed to examine the distribution of mast cells and antimicrobial peptides, including human β-defensin-1 and -2 and LL-37, in the external auditory canal skin.
External auditory canal skin samples from 12 patients undergoing middle-ear surgery with canaloplasty were immunohistochemically stained to detect expression of mast cell markers (tryptase and chymase) and antimicrobial peptides (human β-defensin-1 and -2 and LL-37).
Mast cells and human β-defensin-1 were present in the ceruminous glands but not in the sebaceous glands. The increased presence of mast cells, human β-defensin-1 and LL-37 in ceruminous glands suggests that mast cells may participate in the secretion of antimicrobial peptides from ceruminous glands.
These findings suggest that mast cells contribute to the secretion of antimicrobial peptides in the ceruminous glands of the external auditory canal skin.
抗菌肽对微生物的直接活性被认为是皮肤的重要第一道防线；然而，关于外耳道皮肤中抗菌肽的分泌情况却知之甚少。有证据表明肥大细胞有助于抗菌肽的分泌。本研究旨在检测肥大细胞和抗菌肽，包括人β-防御素-1、β-防御素-2和LL-37，在外耳道皮肤中的分布。
对12例行中耳手术并行外耳道成形术患者的外耳道皮肤样本进行免疫组织化学染色，以检测肥大细胞标志物（类胰蛋白酶和糜酶）和抗菌肽（人β-防御素-1、β-防御素-2和LL-37）的表达。
肥大细胞和人β-防御素-1存在于耵聍腺中，而皮脂腺中不存在。肥大细胞、人β-防御素-1和LL-37在耵聍腺中含量增加，提示肥大细胞可能参与了耵聍腺抗菌肽的分泌。
这些发现表明肥大细胞有助于外耳道皮肤耵聍腺中抗菌肽的分泌。
