Yoon Yong Joo, Yoon Jin, Lee Eun Jung, Kim Jong Seung
Department of Otolaryngology, Chonbuk National University Medical School and Research Institute of Chonbuk National University Hospital, Jeonju, Korea.
Department of Otolaryngology, Wonkwang University Hospital, Iksan, Korea.
Clin Exp Otorhinolaryngol. 2017 Dec;10(4):321-324. doi: 10.21053/ceo.2017.00129. Epub 2017 Sep 5.
The earwax (cerumen) that covers external auditory canal (EAC) skin contains a mixture of ceruminous and sebaceous gland substances, such as lipids, peptides, and proteins. The components secreted from the ceruminous gland that is a modified sweat gland form cerumen and contain several antimicrobial factors. Since substance P (SP) and calcitonin gene-related peptide (CGRP), known as a secretagogue, have been found in sweat glands, our purpose was to determine the expression of SP and CGRP in the glands of EAC skin.
Sections of normal human EAC skins were immunostained for the presence of SP and CGRP using polyclonal antibodies. Immunoreactivity was detected using an avidin-biotin peroxidase complex method.
SP staining was found in ceruminous gland acini and myoepithelial cells. But the SP staining was not found in the sebaceous glands and epidermal region. CGRP was strongly stained in the ceruminous gland and weakly in the sebaceous gland cells. Interestingly, most prominent staining of SP and CGRP was noted in the myoepithelial cells of the ceruminous gland.
The findings in this study suggest that SP and CGRP are expressed in the glands of the EAC skin and secreted in the process of ceruminous gland secretion.
覆盖外耳道（EAC）皮肤的耳垢（耵聍）包含耵聍腺和皮脂腺分泌物的混合物，如脂质、肽和蛋白质。作为一种改良汗腺的耵聍腺分泌的成分形成耳垢，并含有多种抗菌因子。由于在汗腺中发现了作为促分泌素的P物质（SP）和降钙素基因相关肽（CGRP），我们的目的是确定SP和CGRP在外耳道皮肤腺体中的表达。
使用多克隆抗体对正常人外耳道皮肤切片进行SP和CGRP免疫染色。采用抗生物素蛋白-生物素过氧化物酶复合物法检测免疫反应性。
在耵聍腺腺泡和肌上皮细胞中发现SP染色。但在皮脂腺和表皮区域未发现SP染色。CGRP在耵聍腺中染色强烈，在皮脂腺细胞中染色较弱。有趣的是，在耵聍腺的肌上皮细胞中观察到SP和CGRP最显著的染色。
本研究结果表明，SP和CGRP在外耳道皮肤腺体中表达，并在耵聍腺分泌过程中分泌。
