通过反相离子对高效液相色谱法分离具有翻译活性的信使核糖核酸
Separation of translationally active mRNAs by reversed-phase ion-pair high-performance liquid chromatography.
作者信息
van der Mast C A, Hekstra D, Voorma H O
机构信息
Department of Molecular Cell Biology, University of Utrecht, The Netherlands.
出版信息
J Chromatogr. 1991 Mar 8;564(1):115-25. doi: 10.1016/0378-4347(91)80074-m.
PMID:1860907
Abstract
An ion-pair high-performance liquid chromatographic method on C4 columns was developed for the separation of mRNAs. The addition of methylmercuric hydroxide markedly influenced the separation according to length of these molecules. A method is given to recover minute amounts of translatable mRNA from the organic phase. The resolution of mRNAs improved with increasing pore size of the column support.
摘要
建立了一种在C4柱上的离子对高效液相色谱法用于分离信使核糖核酸（mRNA）。加入氢氧化甲基汞会根据这些分子的长度显著影响分离效果。给出了一种从有机相中回收微量可翻译mRNA的方法。随着柱载体孔径的增加，mRNA的分离度得到改善。
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