Garcia S, Liautard J P
J Chromatogr. 1984 Jul 27;296:355-62. doi: 10.1016/s0021-9673(01)96429-5.
The reversed-phase high-performance liquid chromatography of synthetic and natural RNAs was studied. The results show that base composition alone cannot explain the retention characteristics of natural RNAs; secondary structures are probably involved. However, under the conditions described, the separation was independent of the sizes of the molecules. 18S and 28S ribosomal RNAs were eluted after tRNAs but before 9S globin mRNA. Furthermore, globin mRNA was resolved into two species, one containing and the other lacking poly(A). When applied to the separation of HeLa cell poly(A)-containing RNA, an heterogeneous pattern was obtained. Analysis of peptides synthesized by the mRNA showed that the separation is independent of size.
对合成RNA和天然RNA的反相高效液相色谱法进行了研究。结果表明，仅碱基组成无法解释天然RNA的保留特性；二级结构可能也有影响。然而，在所描述的条件下，分离与分子大小无关。18S和28S核糖体RNA在tRNA之后但在9S珠蛋白mRNA之前洗脱。此外，珠蛋白mRNA被分离成两种，一种含有多聚腺苷酸，另一种不含多聚腺苷酸。当应用于分离HeLa细胞含多聚腺苷酸的RNA时，得到了一种异质模式。对由该mRNA合成的肽的分析表明，分离与大小无关。
