通过气相色谱法和气相色谱-质谱联用技术测定血液中的多硫化物。

Determination of polysulphides in blood by gas chromatography and gas chromatography-mass spectrometry.

作者信息

Kage S, Nagata T, Kudo K

机构信息

Department of Forensic Medicine, Faculty of Medicine, Kyushu University, Japan.

出版信息

J Chromatogr. 1991 Mar 8;564(1):163-9. doi: 10.1016/0378-4347(91)80078-q.

DOI:10.1016/0378-4347(91)80078-q

PMID:1860910

Abstract

A sensitive and simple method to determine polysulphides in human blood, using an extractive alkylation technique and gas chromatography, has been devised. Polysulphides were alkylated with pentafluorobenzyl bromide, and then converted into bis(pentafluorobenzyl)disulphide by desulphuration with potassium cyanide. The disulphide was analysed qualitatively by mass fragmentography and quantitatively by gas chromatography with electron-capture detection. The lower limit of detection was 0.005 mumol/ml. Field testing in a suicide case confirmed the validity of the method.

摘要

已设计出一种灵敏且简便的方法，利用萃取烷基化技术和气相色谱法测定人血中的多硫化物。多硫化物用五氟苄基溴进行烷基化，然后通过用氰化钾脱硫转化为双（五氟苄基）二硫化物。通过质量碎片分析法对二硫化物进行定性分析，并用带有电子捕获检测的气相色谱法进行定量分析。检测下限为0.005微摩尔/毫升。在一个自杀案例中的现场测试证实了该方法的有效性。