Chang Young Woon, Oh Hyoung-Chul, Jang Jae Young, Hwangbo Young, Lee Jae Won, Lee Hyo Jung, Joo Kwang Ro, Dong Seok Ho, Kim Sung Soo, Kim Hyo Jong, Kim Byung Ho, Chang Rin
Department of Gastroenterology, Kyung Hee University College of Medicine, Seoul, Korea.
Scand J Gastroenterol. 2008;43(10):1184-93. doi: 10.1080/00365520802130209.
Relations between host genetic factors and clinical outcomes of Helicobacter pylori infection are variable among ethnicities. The aim of this study was to examine gastric mucosal cytokines, matrix metalloproteinase 3 (MMP-3), and serum pepsinogen levels before and after eradication of H. pylori according to IL-1B genotypes and benign gastroduodenal phenotypes in a Korean population.
A total of 349 Koreans including H. pylori-infected subjects (n=230) and H. pylori-negative controls (n=119) were enrolled. The former subjects were classified into groups according to the presence of non-atrophic gastritis (n=74), atrophic gastritis (n=56), gastric ulcer (n=37), and duodenal ulcer (n=63). IL-1B polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Gastric mucosal IL-1beta, IL-8, and MMP-3, and serum pepsinogen I and II levels were measured by ELISA and radioimmunoassay, respectively.
There were no significant differences between the IL-1B-31/-511 haplotype (TT/CC, CT/CT, and CC/TT) frequencies among the H. pylori-positive and -negative groups. The genotypes of IL-1B-31/-511 polymorphisms did not affect clinical phenotypes, inflammatory cytokines, MMP-3, and pepsinogen secretion. Subjects with H. pylori-infected atrophic gastritis exhibited significantly higher basal levels of cytokines and a lower pepsinogen I/II ratio than those of other groups. Following H. pylori eradication, inflammatory cytokines significantly decreased and the pepsinogen I/II ratio increased in all groups.
Mucosal inflammatory cytokines, MMP-3, and pepsinogen secretion are related to gastroduodenal phenotypes but not to IL-1B genotypes. Eradication of H. pylori can reduce mucosal inflammation and restore pepsinogen secretion.
宿主遗传因素与幽门螺杆菌感染临床结局之间的关系在不同种族中存在差异。本研究旨在根据白细胞介素-1β（IL-1β）基因型和良性胃十二指肠表型，检测韩国人群中幽门螺杆菌根除前后胃黏膜细胞因子、基质金属蛋白酶3（MMP-3）和血清胃蛋白酶原水平。
共纳入349名韩国人，包括幽门螺杆菌感染患者（n=230）和幽门螺杆菌阴性对照者（n=119）。前者根据是否存在非萎缩性胃炎（n=74）、萎缩性胃炎（n=56）、胃溃疡（n=37）和十二指肠溃疡（n=63）进行分组。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对IL-1β多态性进行基因分型。分别采用酶联免疫吸附测定（ELISA）和放射免疫测定法检测胃黏膜IL-1β、IL-8和MMP-3以及血清胃蛋白酶原I和II水平。
幽门螺杆菌阳性和阴性组之间IL-1β -31/-511单倍型（TT/CC、CT/CT和CC/TT）频率无显著差异。IL-1β -31/-511多态性的基因型不影响临床表型、炎性细胞因子、MMP-3和胃蛋白酶原分泌。幽门螺杆菌感染的萎缩性胃炎患者的细胞因子基础水平显著高于其他组，而胃蛋白酶原I/II比值则较低。根除幽门螺杆菌后，所有组的炎性细胞因子均显著降低，胃蛋白酶原I/II比值升高。
黏膜炎性细胞因子、MMP-3和胃蛋白酶原分泌与胃十二指肠表型有关，但与IL-1β基因型无关。根除幽门螺杆菌可减轻黏膜炎症并恢复胃蛋白酶原分泌。
