Department of Chemical Engineering, Princeton University Princeton, NJ 08544-5263, USA.
Biotechnol Bioeng. 1993 Feb 5;41(3):341-6. doi: 10.1002/bit.260410308.
Gas antisolvent (GAS) expansion of dimethylsulfoxide (DMSO) and N,N-dimethylformamide (DMFA) solutions with supercritical carbon dioxide was used to produce biologically active powders of insulin. Powders with 90% of the particles smaller than 4 mum and 10% smaller than 1 mum were obtained under all conditions tested when the process was operated continuously, with small liquid droplets sprayed into a flowing supercritical continuum. Slow pressurization of the stagnant protein solution resulted in larger particles. In vivo tests on rats revealed no differences between the biological activity of processed and unprocessed insulin, GAS processing of organic solution appears to be a reliable and effective method for the production of dry, biologically active microparticulate powders of peptides and proteins.
气体抗溶剂（GAS）法联合超临界二氧化碳对二甲亚砜（DMSO）和 N,N-二甲基甲酰胺（DMFA）溶液进行膨胀，从而制备具有生物活性的胰岛素粉末。当连续操作且液体以小液滴的形式喷射到超临界连续相中时，在所有测试条件下都可以得到粒径 90%小于 4 µm，10%小于 1 µm 的粉末。当蛋白质溶液在静态下缓慢加压时，会得到更大的颗粒。在大鼠体内试验中，处理过和未处理过的胰岛素的生物活性没有差异，表明有机溶液的 GAS 处理是一种可靠且有效的方法，可用于制备干燥的、具有生物活性的肽和蛋白质的微粒粉末。
