Biochemical Engineering Laboratory, MOGAM Biotechnology Research Institute, Yongin-Goon Kyounggi-Do 449-910, Republic of Korea.
Biotechnol Bioeng. 1993 Nov 20;42(10):1218-28. doi: 10.1002/bit.260421012.
A series of high-density media for mammalian cell culture were developed by step-fortifications of most nutrient components in RPMI-1640 medium. Each medium constituting the series was constructed to meet in vitro cell growth limitations. Four different cell lines were cultivated in the media series, and their growth characteristics were observed. Maximum cell densities varied in the range of 0.4 to 1.3 x 10(7) cells/mL, depending on cell lines. Cell growth responses to each of the media series were analyzed in terms of cell density and cell mass. Step increases of cell mass in the range of 1.3 to 3.7 g/L were observed according to the step-fortifications of nutrients. Also, the characteristics of each cell line were compared in terms of metabolic yields and specific productions of lactic acid and ammonium ion. The effect of step-fortifications of nutrients on the production of monoclonal antibody was also examined. Apparent differences in metabolic characteristics among cell lines were observed. Experimental results suggested that the different cell sizes and metabolic characteristics of each cell line resulted in cell-line-specific responses to the step-fortifications. The significant influence of nutritional fortifications on high-density culture of mammalian cells was evaluated.
一系列用于哺乳动物细胞培养的高密度培养基是通过逐步强化 RPMI-1640 培养基中的大多数营养成分而开发的。该系列中的每一种培养基都是为了满足体外细胞生长的限制而构建的。在该培养基系列中培养了四种不同的细胞系，并观察了它们的生长特性。最大细胞密度根据细胞系的不同，在 0.4 到 1.3 x 10(7)个细胞/ml 之间变化。根据营养成分的逐步强化，分析了细胞密度和细胞质量对每个培养基系列的细胞生长反应。观察到细胞质量根据营养成分的逐步强化而增加了 1.3 到 3.7 g/L 的范围。此外，还比较了每个细胞系的代谢产率和乳酸及铵离子的比生产特性。还检查了营养成分的逐步强化对单克隆抗体生产的影响。观察到细胞系之间代谢特性的明显差异。实验结果表明，不同细胞系的细胞大小和代谢特性导致了对营养成分逐步强化的特定细胞系反应。评估了营养强化对哺乳动物细胞高密度培养的显著影响。
