Ondrej Vladan, Lukásová Emilie, Krejcí Jana, Matula Pavel, Kozubek Stanislav
Laboratory of Molecular Cytology and Cytometry, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska, Brno, Czech Republic.
Mol Cells. 2008 Oct 31;26(4):356-61. Epub 2008 Jul 9.
In this work, we have studied the structural and functional linkage between lamin A/C, nuclear actin, and organization of chromosome territories (CTs) in mammary carcinoma MCF-7 cells. Selective down-regulation of lamin A/C expression led to disruption of the lamin A/C perinuclear layer and disorganization of lamin-bound emerin complexes at the inner nuclear membrane. The silencing of lamin A/C expression resulted in a decrease in the volume and surface area of chromosome territories, especially in chromosomes with high heterochromatin content. Inhibition of actin polymerization led to relaxation of the structure of chromosome territories, and an increase in the volumes and surface areas of the chromosome territories of human chromosomes 1, 2 and 13. The results show an important role of polymeric actin in the organization of the nuclei and the chromosome territories.
在这项工作中，我们研究了乳腺癌MCF-7细胞中核纤层蛋白A/C、核肌动蛋白与染色体区域（CTs）组织之间的结构和功能联系。核纤层蛋白A/C表达的选择性下调导致核纤层蛋白A/C核周层的破坏以及内核膜上与核纤层结合的emerin复合物的紊乱。核纤层蛋白A/C表达的沉默导致染色体区域的体积和表面积减小，尤其是在异染色质含量高的染色体中。肌动蛋白聚合的抑制导致染色体区域结构的松弛，以及人类染色体1、2和13的染色体区域的体积和表面积增加。结果表明聚合肌动蛋白在细胞核和染色体区域的组织中起重要作用。
