三种淋巴母细胞系酶谱与一种新的微量技术的比较。

Comparison of zymogram of three lymphoblastoid cell lines with a new microtechnique.

作者信息

Nardon P, Monget D, Didier-Fichet M L, de-Thé G

出版信息

Biomedicine. 1976 Jun;24(3):183-90.

Abstract

The enzymatic activities of three lymphoblastoid cell lines were subjected to a comparative analysis of 22 enzymatic activities with a new microtechnique "Apizym". This method is easy, requires only a small number of cells and allows simultaneous testing of many enzymatic activities from the same sample. The three cell lines can be easily distinguished despite their close origin: NAB, derived from a malignant retroperitoneal mass in an American patient, is the cell line with the greatest total activity and HRlK (a clone of P3J, derived from an African lymphoma) with the lowest and RAJI (a non-virus producing line derived from an African lymphoma) being the intermediate. The more striking differences concern the activity of phosphatases (NAB greater than RAJI greater than or equal to HRlK), esterases-lipases (NAB greater than or equal to RAJI greater than HRlK), beta glucosaminidase, beta glucuronidase beta glucosidase (NAB greater than or equal to RAJI greater than HRlK). On the other hand, alpha mannosidase and alpha fucosidase are more active in RAJI (RAJI greater than NAB greater than or equal to HRlK).

摘要

采用新的微量技术“ApiZym”对三种淋巴母细胞系的酶活性进行了22种酶活性的比较分析。该方法简便，仅需少量细胞，且能对同一样本的多种酶活性进行同步检测。尽管这三种细胞系起源相近，但仍能轻易区分：NAB源自一名美国患者的恶性腹膜后肿块，是总活性最高的细胞系；HRlK（源自非洲淋巴瘤的P3J克隆）活性最低；RAJI（源自非洲淋巴瘤的非病毒产生系）活性居中。更为显著的差异在于磷酸酶活性（NAB＞RAJI≥HRlK）、酯酶 - 脂肪酶活性（NAB≥RAJI＞HRlK）、β - 氨基葡萄糖苷酶、β - 葡萄糖醛酸酶、β - 葡萄糖苷酶活性（NAB≥RAJI＞HRlK）。另一方面，α - 甘露糖苷酶和α - 岩藻糖苷酶在RAJI中活性更高（RAJI＞NAB≥HRlK）。