利用酶谱快速鉴定淋病奈瑟菌和脑膜炎奈瑟菌。
Rapid identification of Neisseria gonorrhoeae and Neisseria meningitidis by using enzymatic profiles.
作者信息
D'Amato R F, Eriquez L A, Tomfohrde K M, Singerman E
出版信息
J Clin Microbiol. 1978 Jan;7(1):77-81. doi: 10.1128/jcm.7.1.77-81.1978.
Abstract
The enzymatic profiles of Neisseria gonorrhoeae, N. meningitidis, and related species were determined, using a total of 48 chromogenic substrates. Enzyme classes assayed for included glycosidases, aminopeptidases, phosphoamidases, proteases, lipases, esterases, and aryl sulfatase. A final test selection of 10 substrates, based upon their differential and reproducible characteristics, allowed the separation of N. gonorrhoeae and N. meningitidis from each other and from all species tested within 4 h after primary isolation on modified Thayer-Martin medium. The need for subculturing suspect colonies from modified Thayer-Martin medium to chocolate medium with a subsequent loss of 18 to 24 h of identification is eliminated.
摘要
使用总共48种显色底物测定了淋病奈瑟菌、脑膜炎奈瑟菌及相关菌种的酶谱。检测的酶类包括糖苷酶、氨肽酶、磷酸酰胺酶、蛋白酶、脂肪酶、酯酶和芳基硫酸酯酶。基于10种底物的差异和可重复性特征进行的最终测试选择，能够在改良的Thayer-Martin培养基上初次分离后4小时内，将淋病奈瑟菌和脑膜炎奈瑟菌彼此区分开，并与所有测试菌种区分开来。这样就无需将改良的Thayer-Martin培养基上的可疑菌落转种至巧克力培养基，从而避免了后续鉴定过程中18至24小时的时间损失。
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