Tseng Guan-Chin, Peng Yen-Chun, Wang Hsuan-Hui, Lee Fu-Hui, Huang Lan-Ru
Department of Pathology, China Medical University Hospital, Taichung, Taiwan.
Hepatogastroenterology. 2008 Mar-Apr;55(82-83):535-8.
BACKGROUND/AIMS: Matrix metalloproteinases 2 and 9 (MMP-2 and -9) have been suggested to play an important role in hepatitis and hepatic fibrosis. The aims of this study were to determine the plasma levels of enzyme activities of matrix metalloproteinases 2 and 9 in healthy and deferred blood donors. The relationships between activities of matrix metalloproteinases 2 and 9 and the level of alanine aminotransferase and the status of hepatitis B virus and hepatitis C virus infection were investigated.
This study included 51 healthy volunteer blood donors and 175 deferred blood donors. Of the deferred donors, 54 donors had levels of alanine aminotransferase > or = 45 IU/L, 99 donors were positive for hepatitis B virus surface antigen, and 32 donors were positive for antibodies against hepatitis C virus. Activities of matrix metalloproteinases 2 and 9 were measured by zymography using impregnated gel.
Activity of matrix metalloproteinase 2 in deferred blood donors with elevated ALT was higher than that in healthy donors (331 +/- 139 vs. 266 +/- 122, p < 0.001). Further, activity of matrix metalloproteinase 2 was higher in HCV-infected donors than in healthy donors (319 +/- 97 vs. 275 +/- 133, p < 0.05). Matrix metalloproteinase 9 activity was also higher in deferred donors with HCV infection than in healthy donors (153 +/- 45 vs. 108 +/- 48, p < 0.001).
These findings suggest that hepatitis C virus infection may upregulate the activities of matrix metalloproteinases 2 and 9 and that these matrix metalloproteinases may be linked to liver dysfunction.
背景/目的：有研究表明基质金属蛋白酶2和9（MMP - 2和 - 9）在肝炎和肝纤维化中发挥重要作用。本研究旨在测定健康献血者和延期献血者血浆中基质金属蛋白酶2和9的酶活性水平。研究了基质金属蛋白酶2和9的活性与丙氨酸氨基转移酶水平以及乙型肝炎病毒和丙型肝炎病毒感染状况之间的关系。
本研究纳入了51名健康志愿者献血者和175名延期献血者。在延期献血者中，54名献血者丙氨酸氨基转移酶水平≥45 IU/L，99名献血者乙型肝炎病毒表面抗原呈阳性，32名献血者丙型肝炎病毒抗体呈阳性。采用含浸凝胶酶谱法测定基质金属蛋白酶２和9的活性．
丙氨酸氨基转移酶升高的延期献血者中基质金属蛋白酶2的活性高于健康献血者（331±139对266±122，p<0.001）。此外，丙型肝炎病毒感染的献血者中基质金属蛋白酶2的活性高于健康献血者（319±97对275±133，p<0.05）。丙型肝炎病毒感染的延期献血者中基质金属蛋白酶9的活性也高于健康献血者（153±45对108±48，p<0.001）。
这些发现表明丙型肝炎病毒感染可能上调基质金属蛋白酶2和9的活性，并且这些基质金属蛋白酶可能与肝功能障碍有关。
