Lee-Young Robert S, Koufogiannis George, Canny Benedict J, McConell Glenn K
Department of Physiology, The University of Melbourne, Parkville, Victoria, Australia.
Med Sci Sports Exerc. 2008 Aug;40(8):1490-4. doi: 10.1249/MSS.0b013e318173a037.
No study has examined the response of skeletal muscle AMP-activated protein kinase (AMPK) signaling beyond the first 3 h after an acute exercise bout in humans. The purpose of this study was to assess AMPK signaling in human skeletal muscle immediately after a single bout of moderate-intensity endurance exercise and 3 and 24 h after the exercise bout.
We examined AMPK signaling, and protein expression of AMPK alpha, ACC-beta, and nNOS mu in untrained individuals (four females and four males) during the 24-h period after a 60-min bout of moderate-intensity (63 +/- 1% VO2peak) cycling endurance exercise.
AMPK alpha2 activity, AMPK alpha2 Thr172 phosphorylation, and ACC-beta Ser222 phosphorylation were increased immediately after exercise. These increases had all returned to basal levels at 3 and 24 h after exercise. Furthermore, an acute bout of exercise did not alter AMPK alpha1, AMPK alpha2, ACC-beta, or nNOS mu protein expression during the 24-h period after exercise.
Although an acute bout of exercise elicits increases in AMPK signaling, this alone is not sufficient to induce sustained increases in either AMPK signaling or protein expression during the postexercise period.
尚无研究在人体急性运动发作后的最初3小时之后检测骨骼肌AMP激活蛋白激酶（AMPK）信号通路的反应。本研究的目的是评估单次中等强度耐力运动后即刻以及运动后3小时和24小时时人体骨骼肌中的AMPK信号通路。
我们在60分钟中等强度（63±1%VO₂峰值）骑行耐力运动后的24小时内，检测了未受过训练的个体（4名女性和4名男性）的AMPK信号通路以及AMPKα、ACC-β和nNOSμ的蛋白表达。
运动后即刻，AMPKα2活性、AMPKα2苏氨酸172磷酸化以及ACC-β丝氨酸222磷酸化增加。这些增加在运动后3小时和24小时时均恢复至基础水平。此外，一次急性运动发作在运动后的24小时内未改变AMPKα1、AMPKα2、ACC-β或nNOSμ的蛋白表达。
尽管一次急性运动发作会引起AMPK信号通路增加，但仅此一点不足以在运动后期间诱导AMPK信号通路或蛋白表达的持续增加。
