MacKenzie Donald A, Defernez Marianne, Dunn Warwick B, Brown Marie, Fuller Linda J, de Herrera Santiago R M Seco, Günther Andreas, James Steve A, Eagles John, Philo Mark, Goodacre Royston, Roberts Ian N
National Collection of Yeast Cultures, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
Yeast. 2008 Jul;25(7):501-12. doi: 10.1002/yea.1601.
Ten medically important Saccharomyces strains, comprising six clinical isolates of Saccharomyces cerevisiae and four probiotic strains of Saccharomyces boulardii, were characterized at the genetic and metabolic level and compared with non-medical, commercial yeast strains used in baking and wine-making. Strains were compared by genetic fingerprinting using amplified fragment length polymorphism (AFLP) analysis, by ribosomal DNA ITS1 sequencing and by metabolic footprinting using both direct injection mass spectrometry (DIMS) and gas chromatography-time of flight-mass spectrometry (GC-ToF-MS). Overall, the clinical isolates fell into different groupings when compared with the non-medical strains, with good but not perfect correlation amongst strains at both the genetic and metabolic levels. Probiotic strains of S. boulardii that are used therapeutically to treat human gastro-intestinal tract disorders showed tight clustering both genetically and metabolically. Metabolomics was found to be of value both as a taxonomic tool and as a means to investigate anomalous links between genotype and phenotype. Key discriminatory metabolites were identified when comparing the three main groups of clinical, probiotic and non-medical strains and included molecules such as trehalose, myo-inositol, lactic acid, fumaric acid and glycerol 3-phosphate. This study confirmed the link between a subset of clinical isolates and baking or probiotic strains but also highlighted that in general the clinical strains were more diverse at both the genomic and metabolic levels.
对10株具有医学重要性的酵母菌株进行了遗传和代谢水平的表征，其中包括6株酿酒酵母临床分离株和4株布拉酵母菌益生菌株，并将它们与用于烘焙和酿酒的非医用商业酵母菌株进行了比较。通过使用扩增片段长度多态性（AFLP）分析的遗传指纹图谱、核糖体DNA ITS1测序以及使用直接进样质谱（DIMS）和气相色谱-飞行时间质谱（GC-ToF-MS）的代谢足迹分析对菌株进行比较。总体而言，与非医用菌株相比，临床分离株分为不同的组，在遗传和代谢水平上菌株之间具有良好但并非完美的相关性。用于治疗人类胃肠道疾病的布拉酵母菌益生菌株在遗传和代谢上均表现出紧密的聚类。代谢组学被发现作为一种分类工具以及研究基因型和表型之间异常联系的手段均具有价值。在比较临床、益生菌和非医用菌株这三个主要组时鉴定出了关键的鉴别性代谢物，包括海藻糖、肌醇、乳酸、富马酸和3-磷酸甘油等分子。这项研究证实了一部分临床分离株与烘焙或益生菌株之间的联系，但也强调了一般而言临床菌株在基因组和代谢水平上更加多样化。
