犬乳腺肿瘤中VEGF-C表达的定量分析。
Quantification of VEGF-C expression in canine mammary tumours.
作者信息
Qiu C, Lin D D, Wang H H, Qiao C H, Wang J, Zhang T
机构信息
College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
出版信息
Aust Vet J. 2008 Jul;86(7):279-82. doi: 10.1111/j.1751-0813.2008.00312.x.
BACKGROUND
Tumours release angiogenic factors such as vascular endothelium growth factor (VEGF), which induces growth of a capillary network around the tumour. Elevated concentrations of VEGF have been reported in human mammary gland tumours.
METHODS
To evaluate the expression of VEGF-C mRNA in canine mammary tissue, 38 mammary gland tumours (including 15 benign and 23 malignant mammary tumours), and 4 normal mammary glands were investigated by real-time reverse transcriptase quantitative polymerase chain reaction.
RESULTS
VEGF-C expression in the malignant mammary tumours was much higher than in the benign mammary tumours or normal mammary tissue (P < 0.001). The expression of VEGF-C in tumours with lymph node metastasis was much higher than in those without (P < 0.01).
CONCLUSION
The level of expression of VEGF-C did not correlate with tumour size or the patient's age, but was significantly higher in malignant mammary tumours and related to lymph node metastasis, making it a candidate marker for predicting metastasis of canine mammary cancer.
背景
肿瘤会释放血管生成因子，如血管内皮生长因子（VEGF），其可诱导肿瘤周围毛细血管网络的生长。据报道，人乳腺肿瘤中VEGF浓度升高。
方法
为评估VEGF-C mRNA在犬乳腺组织中的表达，采用实时逆转录定量聚合酶链反应对38个乳腺肿瘤（包括15个良性和23个恶性乳腺肿瘤）及4个正常乳腺组织进行研究。
结果
恶性乳腺肿瘤中VEGF-C的表达远高于良性乳腺肿瘤或正常乳腺组织（P<0.001）。有淋巴结转移的肿瘤中VEGF-C的表达远高于无淋巴结转移的肿瘤（P<0.01）。
结论
VEGF-C的表达水平与肿瘤大小或患者年龄无关，但在恶性乳腺肿瘤中显著升高且与淋巴结转移有关，使其成为预测犬乳腺癌转移的候选标志物。
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