Lin Y F, Jing W, Wu L, Li X Y, Wu Y, Liu L, Tang W, Long J, Tian W D, Mo X M
State Key Laboratory of Oral Diseases, Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, China.
Cell Prolif. 2008 Oct;41(5):803-12. doi: 10.1111/j.1365-2184.2008.00542.x.
In this study, a group of cells that expressed both osteogenic and adipogenic characters was identified from murine adipose stromal cells.
These cells could be enriched in the Sca-1-1 population and express both osteogenic and adipogenic genes. Osteogenic induction enhanced expression of osteogenic genes and inhibited expression of adipogenic genes, while adipogenic induction enhanced expression of adipogenic genes and inhibited expression of osteogenic genes. These cells have been called osteo-adipo progenitors (OAPs).
OAPs expressed transcription factor runt-related transcription factor 2 (RUNX2) and peroxisome proliferator-activated receptor-gamma (PPAR-gamma) proteins in cytoplasm. When OAPs were cultured in adipogenic medium, PPAR-gamma moved to the nucleus and the cells differentiated into adipocytes, while the RUNX2 remained in the cytoplasm. In contrast, when OAPs were cultured in osteogenic medium, RUNX2 moved to the nucleus and the cells differentiated to osteocytes, while the PPAR-gamma remained in the cytoplasm.
These experiments suggest that osteoblasts and adipocytes share a common predecessor, the OAP, in murine adipose stromal cells.
在本研究中，从鼠脂肪基质细胞中鉴定出一组同时具有成骨和成脂特性的细胞。
这些细胞可在Sca-1-1群体中富集，并同时表达成骨和成脂基因。成骨诱导增强成骨基因的表达并抑制成脂基因的表达，而成脂诱导则增强成脂基因的表达并抑制成骨基因的表达。这些细胞被称为骨脂祖细胞（OAPs）。
OAPs在细胞质中表达转录因子 runt相关转录因子2（RUNX2）和过氧化物酶体增殖物激活受体γ（PPAR-γ）蛋白。当OAPs在成脂培养基中培养时，PPAR-γ转移至细胞核，细胞分化为脂肪细胞，而RUNX2仍留在细胞质中。相反，当OAPs在成骨培养基中培养时，RUNX2转移至细胞核，细胞分化为骨细胞，而PPAR-γ仍留在细胞质中。
这些实验表明，在鼠脂肪基质细胞中，成骨细胞和脂肪细胞有一个共同的前身，即OAP。
