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双壳贝类软体动物Aulacomya ater精子细胞核中染色质的核小体组织。

Nucleosomal organization of chromatin in sperm nuclei of the bivalve mollusc Aulacomya ater.

作者信息

Olivares C, Ruiz S

机构信息

Laboratory of Developmental Biology, Universidad de Talca, Chile.

出版信息

Mol Cell Biochem. 1991 Mar 13;101(2):93-9. doi: 10.1007/BF00229527.

Abstract

The sperm nuclei of Aulacomya ater, family Mitylidae, contain three proteins (X, Aa5 and Aa6) which are specific to this cell type coexisting with a set of five somatic-type histones. Information about the chromatin structure resulting from this kind of association is scarce. Therefore, we have probed the structure of this sperm chromatin through digestion with micrococcal nuclease in combination with salt fractionation. The data obtained have allowed us to propose a nucleosomal arrangement for this chromatin. However, two types of nucleosomes would be present in agreement with their protein components.

摘要

贻贝科的黑壳菜蛤精子细胞核含有三种特定于该细胞类型的蛋白质(X、Aa5和Aa6),它们与一组五种体细胞型组蛋白共存。关于这种关联所产生的染色质结构的信息很少。因此,我们通过微球菌核酸酶消化结合盐分级分离来探究这种精子染色质的结构。所获得的数据使我们能够提出这种染色质的核小体排列方式。然而,根据其蛋白质成分,会存在两种类型的核小体。

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