Ausio J, Van Holde K E
Eur J Biochem. 1987 Jun 1;165(2):363-71. doi: 10.1111/j.1432-1033.1987.tb11449.x.
Most of the DNA in the sperm of the bivalve mollusc. Spisula solidissima, is found to be associated with a specific high-molecular-mass, protamine-like component, sharing features common both to protamines and to histones. We have found that this component coexists, in the mature sperm nucleus, with a complete set of histones, including an H1-like histone. Such histones account for approximately 20% of the whole protein content in the sperm chromatin, the overall protein/DNA ratio (w/w) being 0.87. These data, together with micrococcal nuclease digestions in combination with salt fractionation, have allowed us to propose a structural model for this chromatin in which short nucleosomal domains are interspersed in a highly saturated protamine-DNA complex.
在双壳贝类软体动物——坚固团扇蛤的精子中，大部分DNA被发现与一种特定的高分子质量、类鱼精蛋白成分相关联，该成分兼具鱼精蛋白和组蛋白的共同特征。我们发现，在成熟精子细胞核中，这种成分与一整套组蛋白共存，其中包括一种类H1组蛋白。此类组蛋白约占精子染色质中总蛋白含量的20%，总的蛋白质/DNA比率（重量/重量）为0.87。这些数据，连同微球菌核酸酶消化与盐分级分离相结合的结果，使我们能够提出这种染色质的结构模型，其中短核小体结构域散布于高度饱和的鱼精蛋白-DNA复合物中。
