Gabella G, Raeymaekers L
Cell Tissue Res. 1976 Oct 1;173(1):29-44. doi: 10.1007/BF00219264.
The effect of the enzyme collagenase (40-200 units-ml-1) on the spontaneous mechanical activity in vitro and on the fine structure of the activity of the taenia was enhanced both in the isometric and isotonic recordings; after several minutes the muscles became slack or elongated to up to twice their resting lengths. The structural changes were dramatic but a number of muscle cells remained apparently unaltered even with the highest concentration and the longest incubation time (120 minutes). The large variety of structural changes were tentatively grouped into two separate sequences. One sequence involved swelling of the muscle cell, dispersion of the filaments and breaking up of the cell membrane: the thick myofilaments increased considerably in size and became heterogeneous in size and shape, but were still recognizable after disruption of the cell membrane. The other disruptive sequence involved separation of the superficial part of the muscle cell, which became electron-lucent, from the core of the cell where filaments were very densely packed. Few or no changes were observed in non-muscle cells.
胶原酶（40 - 200单位·毫升⁻¹）对等长和等张记录中体外自发机械活动以及绦虫活动精细结构的影响均增强；几分钟后，肌肉松弛或伸长至静息长度的两倍。结构变化显著，但即使在最高浓度和最长孵育时间（120分钟）下，仍有许多肌肉细胞明显未发生改变。大量的结构变化初步分为两个不同的序列。一个序列涉及肌肉细胞肿胀、细丝分散和细胞膜破裂：粗肌丝尺寸显著增加，大小和形状变得不均一，但在细胞膜破裂后仍可识别。另一个破坏序列涉及肌肉细胞表层与细胞核心分离，表层变得电子透明，而细胞核心处细丝非常密集。在非肌肉细胞中观察到很少或没有变化。
