Campbell G R, Chamley J H
Cell Tissue Res. 1975;156(2):201-16. doi: 10.1007/BF00221803.
Smooth muscle cells of the mouse vas deferens fixed with 5% glutaraldehyde contained three types of filaments, namely, thin (50-80) A) filaments, intermediate (100 A) filaments and thick (120-180 A) filments. However, in 2 out of 16 experiments, under identical conditions, the cells did not contain thick filaments. With OSO4 fixation, thin filaments were not prominent, the most obvious being thick (120-250 A) and intermediate (100 A) filaments. After soaking in a modified Ringer solution under no applied tension for one hour, thick filaments (120-180 A) appeared prominently in smooth muscle cells of the mouse vas deferens and thin filaments were in ordered bundles. By 4 hours, thick filaments had increased in size and density, with thin filaments distributed randomly around them. After 8 hours in Ringer, thin filaments were diffuse and difficult to discern, while thick filaments were large (up to 300 A) and electron-dense. Intermediate (100 A) filaments were present in association with dark bodies. Physiological experiments indicated that the intracellular components responsible for the development of a mechanical response were still functional at this time. The presence of "thick filaments" is also reported in degenerating smooth muscle cells of the guinea-pig vas deferens in tissue culture.
用5%戊二醛固定的小鼠输精管平滑肌细胞含有三种类型的细丝，即细（50 - 80）埃的细丝、中间（100埃）细丝和粗（120 - 180埃）细丝。然而，在16次实验中的2次，在相同条件下，细胞不含粗丝。用四氧化锇固定时，细的细丝不明显，最明显的是粗（120 - 250埃）和中间（100埃）细丝。在无外加张力的情况下，在改良林格氏液中浸泡一小时后，粗丝（120 - 180埃）在小鼠输精管平滑肌细胞中显著出现，细的细丝呈有序束状排列。到4小时时，粗丝的大小和密度增加，细的细丝随机分布在它们周围。在林格氏液中浸泡8小时后，细的细丝弥散且难以辨别，而粗丝很大（达300埃）且电子密度高。中间（100埃）细丝与暗体相关存在。生理学实验表明，此时负责产生机械反应的细胞内成分仍有功能。在组织培养中，豚鼠输精管退变的平滑肌细胞中也报道有“粗丝”存在。
