Oh D J, Choi S K, Chang H N
Department of Chemical Engineering and Bioprocess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daeduk Science Town, Taejon 305-701, Korea.
Biotechnol Bioeng. 1994 Oct;44(8):895-901. doi: 10.1002/bit.260440805.
A depth filter perfusion system (DFPS) for animal cell culture was developed and its use in continuous highdensity cultures of hybridoma cells was investigated. In the DFPS, based on cell immobilization in a cylindrical depth filter matrix, cells were easily immobilized and cultivated by simple medium recirculation. The cell density in the 20-mum pore size filter matrix reached up to 3 x 10(7) cells/mLin less than 10 days. This resulted in a high monoclonal antibody productivity of 744 mg/L/day, which was 25-35 times higher than that of continuous-suspension cultures using the same cell line. The 20-mum pore filter retained more cells than the 30-mum filterin a shorter period. The DFPS provides advantages of low-cost set-up, easy operation, and scale-up in the cultures of anchorage-independent cells. It also has a high potential for anchorage-dependent cell cultures because of its unusually high surface-to-volume ratio of 450-600 cm(2)/cm(3). (c) 1994 John Wiley & Sons, Inc.
开发了一种用于动物细胞培养的深层过滤灌注系统（DFPS），并研究了其在杂交瘤细胞连续高密度培养中的应用。在DFPS中，基于细胞固定在圆柱形深层过滤基质中，通过简单的培养基再循环即可轻松固定和培养细胞。在20微米孔径的过滤基质中，细胞密度在不到10天的时间内达到了3×10⁷个细胞/毫升。这导致单克隆抗体的高产率达到744毫克/升/天，比使用相同细胞系的连续悬浮培养高出25至35倍。20微米孔径的过滤器在更短的时间内比30微米的过滤器保留了更多的细胞。DFPS在非贴壁依赖性细胞培养中具有低成本设置、操作简便和易于放大的优点。由于其异常高的450 - 600平方厘米/立方厘米的表面积与体积比，它在贴壁依赖性细胞培养中也具有很高的潜力。（c）1994约翰·威利父子公司。
