Bioengineering Laboratory, Department of Chemical Engineering, State University of New York, Buffalo, NY, 14260, USA.
Cytotechnology. 1999 Jul;30(1-3):133-42. doi: 10.1023/A:1008050809217.
Dielectrophoresis is a well established and effective means for the manipulation of viable cells. However, its effectiveness greatly depends upon the utilization of very low electrical conductivity media. High conductivity media, as in the case of cell culture media, result only in the induction of weaker repulsive forces (negative dielectrophoresis) and excessive medium heating. A dielectrophoresis-based cell separation device (DEP-filter) has been recently developed for perfusion cultures that successfully overcomes these obstacles and provides a very high degree of viable cell separation while most of the nonviable cells are removed from the bioreactor by the effluent stream. The latter results in high viabilities throughout the culture period and minimization of lysed cell proteases in the bioreactor. However, an important question that remains to be answered is whether we have any adverse effects by exposing the cultured cells to high frequency electric fields for extended periods of time. A special chamber was constructed to quantitate the effect under several operational conditions. Cell growth, glucose uptake, lactate and monoclonal antibody production data suggest that there is no appreciable effect and hence, operation over long periods of time of the DEP-filter should not have any adverse effect on the cultured cells.
介电泳是一种成熟且有效的方法，可用于操纵活细胞。然而，其有效性在很大程度上取决于低电导率介质的利用。高电导率介质，如细胞培养基的情况，只会导致较弱的排斥力（负介电泳）的诱导和过度的介质加热。最近开发了一种基于介电泳的细胞分离装置（DEP 过滤器），用于灌注培养，成功克服了这些障碍，在大多数非存活细胞被流出物从生物反应器中去除的同时，提供了非常高的活细胞分离度。后者导致整个培养期间的高存活率，并使生物反应器中的裂解细胞蛋白酶最小化。然而，一个仍然需要回答的重要问题是，长时间暴露于高频电场是否会对培养细胞产生任何不良影响。构建了一个特殊的腔室来在几种操作条件下定量评估这种影响。细胞生长、葡萄糖摄取、乳酸和单克隆抗体生产数据表明，没有明显的影响，因此，DEP 过滤器的长时间操作不应对培养细胞产生任何不良影响。
