Kirschbaum Michael, Jaeger Magnus Sebastian, Schenkel Tim, Breinig Tanja, Meyerhans Andreas, Duschl Claus
Fraunhofer Institute for Biomedical Engineering (IBMT), Lab-On-Chip Technologies, Am Muehlenberg 13, 14476 Potsdam, Germany.
J Chromatogr A. 2008 Aug 15;1202(1):83-9. doi: 10.1016/j.chroma.2008.06.036. Epub 2008 Jul 3.
The gentle and careful in vitro processing of live cells is essential in order to make them available to future therapeutic applications. We present a protocol for the activation of single-T cells based on the contact formation with individual anti-CD3/anti-CD28 presenting microbeads in a lab-on-chip environment. The chips consist of microfluidic channels and microelectrodes for performing dielectrophoretic manipulation employing a.c. electric fields. The dielectrophoretic guiding elements allow the assembly of cell-bead pairs while avoiding ill-defined physical contacts with their environment. After overnight cultivation of the manipulated cells, 77% of the bead-associated T cells expressed the activation marker molecule CD69. Physiological stress on the cells was shown to be mainly due to the single-cell cultivation and not to the manipulation in the chips. The same approach could be useful for the in vitro regulation of stem cell differentiation.
为了使活细胞能够用于未来的治疗应用，对其进行温和且细致的体外处理至关重要。我们提出了一种在芯片实验室环境中基于与单个抗CD3/抗CD28呈递微珠形成接触来激活单个T细胞的方案。这些芯片由微流体通道和微电极组成，用于利用交流电场进行介电泳操作。介电泳引导元件允许细胞 - 微珠对的组装，同时避免与周围环境发生不确定的物理接触。对经操作的细胞进行过夜培养后，77%与微珠结合的T细胞表达了激活标记分子CD69。结果表明，细胞上的生理应激主要是由于单细胞培养，而非芯片中的操作所致。同样的方法可能对干细胞分化的体外调控有用。
