Perrault J, Holland J J
Department of Biology, University of California at San Diego, La Jolla, California 92037, USA.
Virology. 1972 Oct;50(1):159-70. doi: 10.1016/0042-6822(72)90356-x.
Defective interfering particles of New Jersey serotype VSV were found to contain very little or no virion transcriptase activity. Furthermore no VSV transcriptase activity was observed in cells treated with cycloheximide and infected with DI. Cells infected with DI together with standard infectious virus and cycloheximide showed little or no DI interference with standard virion primary transcription, whereas double infection in the absence of cycloheximide exhibited strong DI interference with replication of standard virus genome RNA. We present a model of DI interference based upon competition at the level of viral RNA replication.
新泽西血清型水疱性口炎病毒（VSV）的缺陷干扰颗粒被发现含有极少或没有病毒体转录酶活性。此外，在用环己酰亚胺处理并感染缺陷干扰颗粒的细胞中未观察到VSV转录酶活性。用缺陷干扰颗粒、标准感染性病毒和环己酰亚胺共同感染的细胞对标准病毒体的初级转录几乎没有或没有缺陷干扰颗粒的干扰，而在没有环己酰亚胺的情况下双重感染对标准病毒基因组RNA的复制表现出强烈的缺陷干扰颗粒干扰。我们提出了一个基于病毒RNA复制水平竞争的缺陷干扰颗粒干扰模型。
