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A simple mechanical method to isolate the basal lamina of insect midgut epithelial cells.

作者信息

Koefoed B M

机构信息

University Institute of Pathology, Odense University Hospital, Odense C, Denmark.

出版信息

Tissue Cell. 1985;17(5):763-8. doi: 10.1016/0040-8166(85)90009-6.

DOI:10.1016/0040-8166(85)90009-6
PMID:18620148
Abstract

In mealworms (Tenebrio molitor) the midgut epithelium is surrounded by a 1.6microm thick basal lamina of low electron density with a framework of high electron density imbedded in a part of it. The lamina can be isolated by ultrasonication followed by repeated filtrations and high-speed centrifugations, making large-scale preparation of the lamina for further analyses possible. The isolation of the basal lamina is confirmed by electron microscopy.

摘要

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