通过³H核磁共振监测S-腺苷甲硫氨酸与大肠杆菌DNA腺嘌呤甲基转移酶的变构结合和催化结合。
Allosteric and catalytic binding of S-adenosylmethionine to Escherichia coli DNA adenine methyltransferase monitored by 3H NMR.
作者信息
Bergerat A, Guschlbauer W, Fazakerley G V
机构信息
Département de Biologie Cellulaire et Moléculaire, Centre d'Etudes de Saclay, Gif-sur-Yvette, France.
出版信息
Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6394-7. doi: 10.1073/pnas.88.15.6394.
Abstract
Adenine methylation of GATC sequences in DNA is carried out by the DNA adenine methyltransferase with the methyl group source being the cofactor S-adenosylmethionine. We report 3H NMR studies on the interaction of DNA adenine methyltransferase with S-adenosylmethionine and the reaction when the ternary complex is formed with an oligonucleotide containing a GATC site. The methylation reaction was also studied in the presence of a competitive inhibitor and this showed two successive stages involved in the methylation and two sites of binding for S-adenosylmethionine.
摘要
DNA中GATC序列的腺嘌呤甲基化由DNA腺嘌呤甲基转移酶完成，甲基基团来源为辅因子S-腺苷甲硫氨酸。我们报告了关于DNA腺嘌呤甲基转移酶与S-腺苷甲硫氨酸相互作用的3H NMR研究，以及当三元复合物与含有GATC位点的寡核苷酸形成时的反应。还在存在竞争性抑制剂的情况下研究了甲基化反应，结果表明甲基化过程涉及两个连续阶段以及S-腺苷甲硫氨酸的两个结合位点。
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