Bergerat A, Guschlbauer W, Fazakerley G V
Département de Biologie Cellulaire et Moléculaire, Centre d'Etudes de Saclay, Gif-sur-Yvette, France.
Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6394-7. doi: 10.1073/pnas.88.15.6394.
Adenine methylation of GATC sequences in DNA is carried out by the DNA adenine methyltransferase with the methyl group source being the cofactor S-adenosylmethionine. We report 3H NMR studies on the interaction of DNA adenine methyltransferase with S-adenosylmethionine and the reaction when the ternary complex is formed with an oligonucleotide containing a GATC site. The methylation reaction was also studied in the presence of a competitive inhibitor and this showed two successive stages involved in the methylation and two sites of binding for S-adenosylmethionine.
DNA中GATC序列的腺嘌呤甲基化由DNA腺嘌呤甲基转移酶完成,甲基基团来源为辅因子S-腺苷甲硫氨酸。我们报告了关于DNA腺嘌呤甲基转移酶与S-腺苷甲硫氨酸相互作用的3H NMR研究,以及当三元复合物与含有GATC位点的寡核苷酸形成时的反应。还在存在竞争性抑制剂的情况下研究了甲基化反应,结果表明甲基化过程涉及两个连续阶段以及S-腺苷甲硫氨酸的两个结合位点。