Rabe Kersten S, Gandubert Valérie J, Spengler Mark, Erkelenz Michael, Niemeyer Christof M
Fakultät für Chemie, Biologisch-Chemische Mikrostrukturtechnik, Technische Universität Dortmund, Otto-Hahn-Strabetae 6, 44227, Dortmund, Germany.
Anal Bioanal Chem. 2008 Nov;392(6):1059-73. doi: 10.1007/s00216-008-2248-9. Epub 2008 Jul 13.
Cytochrome P450s constitute a highly fascinating superfamily of enzymes which catalyze a broad range of reactions. They are essential for drug metabolism and promise industrial applications in biotechnology and biosensing. The constant search for cytochrome P450 enzymes with enhanced catalytic performances has generated a large body of research. This review will concentrate on two key aspects related to the identification and improvement of cytochrome P450 biocatalysts, namely the engineering and assaying of these enzymes. To this end, recent advances in cytochrome P450 development are reported and commonly used screening methods are surveyed.
细胞色素P450构成了一个极具吸引力的酶超家族，可催化多种反应。它们对于药物代谢至关重要，并有望在生物技术和生物传感领域得到工业应用。对具有增强催化性能的细胞色素P450酶的不断探索催生了大量研究。本综述将集中于与细胞色素P450生物催化剂的鉴定和改进相关的两个关键方面，即这些酶的工程改造和测定。为此，报告了细胞色素P450开发的最新进展，并对常用的筛选方法进行了调查。
