Li Shun-Xing, Zheng Feng-Ying
Department of Chemistry and Environmental Science, Zhangzhou Normal University, Zhangzhou, P R China.
Planta Med. 2008 Aug;74(10):1302-7. doi: 10.1055/s-2008-1081294. Epub 2008 Jul 11.
Octanol (n-C8H17OH), the configuration of which resembles a carbohydrate and lipid, was used as a biomembrane model, and an octanol-water system was adopted to study the distribution of copper in herbal decoctions under the acidity of saliva, gastric juice, bile and intestinal fluid. Octanol- and water-solubility were used for speciation analysis and the bioavailability assessment of copper. The influence of a combination of Aconitum carmichaeli and Paeonia lactiflora at different ratios, the acidity of the digestive site on the species, and the bioavailability and dose of copper were studied. The concentrations of copper in the original herbal materials and in N-octanol- and water-soluble fractions were determined by flame atomic absorption spectrometry following mixed acid digestion. The results showed that the digestive juice acidity, phytomedical composition, and combination ratio of phytomedicines dominated both the species present and the bioavailability of copper. The concentration of octanol-soluble copper could be used to represent the bioavailability assessment of copper in the phytomedicines, and the dosage of copper in the phytomedicines could be designed based on the level of the octanol-soluble copper and the recommended dietary allowance for copper (0.9 mg/day). Risk assessment could be examined through comparison of the level of octanol-soluble copper and the tolerable upper intake level for copper (10 mg /day).
正辛醇（n-C₈H₁₇OH）的结构类似于碳水化合物和脂质，被用作生物膜模型，采用正辛醇-水体系研究唾液、胃液、胆汁和肠液酸度条件下中药煎剂中铜的分布。利用正辛醇溶解性和水溶性进行铜的形态分析及生物利用度评估。研究了不同比例的附子与白芍组合、消化部位酸度对铜形态、生物利用度及剂量的影响。采用混合酸消解后，通过火焰原子吸收光谱法测定原药材及正辛醇可溶部分和水溶部分中铜的浓度。结果表明，消化液酸度、植物药组成及植物药组合比例主导了铜的存在形态及其生物利用度。正辛醇可溶铜的浓度可用于代表植物药中铜的生物利用度评估，可根据正辛醇可溶铜水平及铜的推荐膳食摄入量（0.9毫克/天）设计植物药中铜的剂量。通过比较正辛醇可溶铜水平与铜的可耐受最高摄入量（10毫克/天）可进行风险评估。
