Shin Richard H, Vathana Torpon, Giessler Goetz A, Friedrich Patricia F, Bishop Allen T, Shin Alexander Y
Department of Orthopedic Surgery, Microvascular Research Laboratory, Mayo Clinic, Rochester, MN 55905, USA.
Microsurgery. 2008;28(6):452-7. doi: 10.1002/micr.20520.
Tetanic force measurements of rat tibialis anterior (TA) muscles have been described, but with a variety of stimulation parameters. This study presents a novel functional method of force measurement of the rat TA muscle and describes the optimization of stimulation parameters. Bilateral TA muscles in 10 male Lewis rats were attached to a force transducer after the corresponding hindlimb was fixed. Preload, stimulus intensity, duration, and frequency were optimized for each individual muscle and the isometric maximal tetanic muscle force was measured. The mean left side tetanic force as a percentage of the right was 100.0 +/- 4.4% and was statistically equivalent. Large standard deviations between sides (35-50%) were observed in the optimized parameters (preload, stimulus intensity, duration, and frequency). Optimization of the variables affecting isometric tetanic force resulted in reproducible and reliable side-to-side measurements of the TA muscle in the rat model.
已有关于大鼠胫前肌（TA）强直力测量的描述，但使用了多种刺激参数。本研究提出了一种测量大鼠TA肌肉力量的新型功能方法，并描述了刺激参数的优化。在固定相应后肢后，将10只雄性Lewis大鼠的双侧TA肌肉连接到力传感器上。针对每块肌肉优化预负荷、刺激强度、持续时间和频率，并测量等长最大强直肌肉力量。左侧强直力占右侧的平均百分比为100.0 +/- 4.4%，在统计学上相等。在优化参数（预负荷、刺激强度、持续时间和频率）中观察到两侧之间存在较大的标准差（35 - 50%）。对影响等长强直力的变量进行优化，使得在大鼠模型中对TA肌肉进行可靠且可重复的双侧测量成为可能。
