Ducret A, Giroux A, Trani M, Lortie R
Bioprocess R&D Group, Biotechnology Research Institute, National Research Council, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2, Canada.
Biotechnol Bioeng. 1995 Nov 5;48(3):214-21. doi: 10.1002/bit.260480308.
Biosurfactants were prepared by enzymatic esterification of sugars and sugar alcohols in nonaqueous media. Sorbitol monooleate was produced in pure molten substrates, with reduced pressure to remove water. The results were compared to synthesis in organic solvent, with and without water removal. Synthesis in organic solvent with water removal, obtained by refluxing through a desiccant under reduced pressure, proved to be the most efficient method in terms of total yield and side-products formation. This process was applied to the production of different surfactants, by changing the nature of the hydrophilic and hydrophobic moieties. Yields above 90% of monoesters were obtained after 24 h when the reaction was carried out in 2-methyl-2-butanol with Novozym 435 (Type B lipase from Candida antarctica) with an excess of hydroxyl donor.
生物表面活性剂是通过在非水介质中对糖类和糖醇进行酶促酯化反应制备的。山梨醇单油酸酯是在纯熔融底物中制备的，通过减压除去水分。将结果与在有或没有除水的有机溶剂中的合成进行比较。通过在减压下通过干燥剂回流获得的在有除水的有机溶剂中的合成，在总产率和副产物形成方面被证明是最有效的方法。通过改变亲水和疏水部分的性质，该方法被应用于不同表面活性剂的生产。当反应在2-甲基-2-丁醇中用诺维信435（来自南极假丝酵母的B型脂肪酶）和过量的羟基供体进行时，24小时后单酯产率高于90%。
