Enzymatic radioiodination of succinyl cyclic AMP tyrosine methyl ester by lactoperoxidase and radioimmunoassay for cyclic AMP.
作者信息
Sato K, Miyachi Y, Mizuchi A, Ohsawa N, Kosaka K
出版信息
Endocrinol Jpn. 1976 Jun;23(3):251-7. doi: 10.1507/endocrj1954.23.251.
PMID:186259
Abstract
A highly specific and simple radioimmunoassay for cyclic AMP with a sensitivity of 0.04 picomoles/tube has been developed according to the method of Steiner et al., using 125I-succinyl cyclic AMP tyrosine methyl ester as a tracer. The tracer with higher immunoactivities could be simply and constantly prepared by an enzymatic iodination procedure utilizing lactoperoxidase, radioactive iodide and hydrogen peroxide generated by glucose-glucose oxidase system, rather than by chloramine-T procedure.
摘要
相似文献
1
Enzymatic radioiodination of succinyl cyclic AMP tyrosine methyl ester by lactoperoxidase and radioimmunoassay for cyclic AMP.
Endocrinol Jpn. 1976 Jun;23(3):251-7. doi: 10.1507/endocrj1954.23.251.
2
Radioimmunoassay for the measurement of adenosine 3',5'-cyclic phosphate.用于测量3',5'-环磷酸腺苷的放射免疫测定法。
Proc Natl Acad Sci U S A. 1969 Sep;64(1):367-73. doi: 10.1073/pnas.64.1.367.
3
A simple ultrasensitive method for the assay of cyclic AMP and cyclic GMP in tissues.
Life Sci. 1976 Mar 1;18(5):529-41. doi: 10.1016/0024-3205(76)90331-3.
4
Attomole sensitive radioimmunoassay for cyclic GMP.用于环磷酸鸟苷的阿托摩尔灵敏放射免疫测定法。
Second Messengers Phosphoproteins. 1988;12(4):145-54.
5
Measurement of 3':5'cyclic AMP in biological samples using a specific monoclonal antibody.使用特异性单克隆抗体测定生物样品中的3':5'环磷酸腺苷
Second Messengers Phosphoproteins. 1988;12(2-3):123-33.
6
7
Preparation of stable 125I cyclic GMP tyrosine methyl ester suitable for 3',5' cyclic GMP radioimmunoassay by HPLC.通过高效液相色谱法制备适用于3',5'-环鸟苷酸放射免疫测定的稳定的125I环鸟苷酸酪氨酸甲酯。
J Immunoassay. 1990;11(1):97-108. doi: 10.1080/01971529008053261.
8
Solid phase radioimmunoassay for cyclic AMP using staphylococcal protein A-antibody adsorbent.使用葡萄球菌蛋白A-抗体吸附剂的环磷酸腺苷固相放射免疫测定法。
Naunyn Schmiedebergs Arch Pharmacol. 1977 May;298(1):67-73. doi: 10.1007/BF00510989.
9
Preparation of high-quality iodine-125-labelled pituitary human follicle-stimulating hormone (hFSH) for radioimmunoassay: comparison of enzymatic and chloramine-T iodination.用于放射免疫分析的高质量碘-125标记垂体人促卵泡激素（hFSH）的制备：酶促碘化与氯胺-T碘化的比较
Clin Chim Acta. 1977 Apr 1;76(1):25-34. doi: 10.1016/0009-8981(77)90115-2.
10
Purification of 125I-labeled succinyl cyclic nucleotide tyrosine methyl esters by high-performance liquid chromatography.通过高效液相色谱法纯化125I标记的琥珀酰环核苷酸酪氨酸甲酯。
Anal Biochem. 1988 Feb 1;168(2):417-20. doi: 10.1016/0003-2697(88)90338-7.
Zotero 插件