两种增强肽（KPP和BPP9a）对激肽诱导的大鼠足爪水肿的比较作用。

Comparative effects of two potentiating peptides (KPP and BPP9a) on kinin-induced rat paw edema.

作者信息

Fernandes P D, Guimarães J A, Assreuy J

机构信息

Departamento de Farmacologia Básica e Clínica, Universidade Federal do Rio de Janeiro, Brasil.

出版信息

Agents Actions. 1991 Mar;32(3-4):182-7. doi: 10.1007/BF01980871.

DOI:10.1007/BF01980871

PMID:1862741

Abstract

We have previously shown that KPP, a kinin potentiating peptide generated by tryptic digestion of human plasma proteins potentiated kinin effects on isolated smooth muscle preparations like guinea-pig ileum with high potency and specificity. We also obtained evidence suggesting that, unlike other potentiating peptides, KPP exerts its effect by a mechanism different from the inhibition of kinin metabolism by angiotensin converting enzyme, neutral endopeptidase and kininase I. Here we show the potentiating effect of KPP and of BPP9a, a potentiator derived from snake venom, towards the rat paw edema induced by bradykinin (BK). Our results show that: a) KPP is 25-fold more active than BPP9a in potentiating rat paw edema elicited by BK: b) like BPP9a, KPP is specific in potentiating kinin-induced edema, being ineffective in potentiating edema induced by histamine or serotonin; and c) DesArg9-BK (DABK) elicits a small edematogenic response which can be potentiated by both KPP and BPP9a.

摘要

我们之前已经表明，激肽增强肽（KPP）是通过对人血浆蛋白进行胰蛋白酶消化产生的一种激肽增强肽，它能高效且特异地增强激肽对离体平滑肌制剂（如豚鼠回肠）的作用。我们还获得了证据，表明与其他增强肽不同，KPP发挥作用的机制不同于通过抑制血管紧张素转换酶、中性内肽酶和激肽酶I对激肽的代谢。在此，我们展示了KPP和BPP9a（一种源自蛇毒的增强剂）对缓激肽（BK）诱导的大鼠足爪水肿的增强作用。我们的结果表明：a）在增强BK引起的大鼠足爪水肿方面，KPP的活性比BPP9a高25倍；b）与BPP9a一样，KPP在增强激肽诱导的水肿方面具有特异性，对组胺或5-羟色胺诱导的水肿无增强作用；c）去精氨酸9-缓激肽（DABK）引发的致水肿反应较小，KPP和BPP9a均可增强该反应。