Devynck M A, Rouzaire-Dubois B, Chevillotte E, Meyer P
Eur J Pharmacol. 1976 Nov;40(1):27-37. doi: 10.1016/0014-2999(76)90350-2.
3H-labelled angiotensin II binding to receptor sites was studied in plasma membranes isolated from myometrial homogenates of uterine horns. Removal of the kidneys, which results in the disappearance of plasma angiotensin II, was followed 19 h after nephrectomy by an increase in the number of uterine receptor sites without significant variation in the apparent dissociation constant. Acute pressor i.v. injection of angiotensin II into nephrectomized rats immediately before removing uteri, did not affect the number of uterine angiotensin receptors, whereas long-lasting angiotensin infusion did reduce the number of receptors. These changes cannot be accounted for by variations in the occupancy of receptor sites. These results demonstrate that the number of angiotensin receptors, at least in uterine contractile cells, is affected by chronic variations of endogenous angiotensin levels. The relation between the specific supersensitivity to angiotensin II observed in uteri from nephrectomized rats and the variations at the receptor level is discussed.
在从子宫角肌层匀浆中分离出的质膜上研究了3H标记的血管紧张素II与受体位点的结合。切除肾脏会导致血浆血管紧张素II消失，肾切除术后19小时，子宫受体位点数量增加，而表观解离常数无显著变化。在切除子宫前立即对肾切除大鼠进行静脉注射血管紧张素II的急性升压注射，不影响子宫血管紧张素受体的数量，而长期输注血管紧张素则会减少受体数量。这些变化不能用受体位点占有率的变化来解释。这些结果表明，血管紧张素受体的数量，至少在子宫收缩细胞中，受内源性血管紧张素水平慢性变化的影响。讨论了在肾切除大鼠子宫中观察到的对血管紧张素II的特异性超敏反应与受体水平变化之间的关系。
