Chevillotte E, Rouzaire-Dubois B, Devynck M A, Meyer P
Contrib Nephrol. 1976;3:134-41. doi: 10.1159/000399390.
3H-labelled angiotensin II binding to receptor sites was studied in plasma membranes isolated from myometrial homogenates of uterine horns. Removal of the kidneys, which results in the disappearance of plasma angiotensin II, was followed by an increase in the number of uterine receptor sites without significant variation in the apparent dissociation constant, which became significant 15 h after nephrectomy. Acute pressor intravenous injection of angiotensin II into nephrectomized rats immediately before removing uteri, did not affect the number of uterine angiotensin receptors, whereas long-lasting angiotensin infusion did reduce the number of receptors. These results provided an explanation for the specific supersensitivity to angiotensin II, observed in uteri excised from nephrectomized rats, which cannot be accounted for by variations in the occupancy of receptor sites. These results also demonstrate that the number of angiotensin receptors, at least in uterine contractile cells, is affected by chronic variations of endogenous angiotensin levels.
在从子宫角肌层匀浆中分离出的质膜中，研究了3H标记的血管紧张素II与受体位点的结合情况。切除肾脏会导致血浆血管紧张素II消失，随后子宫受体位点数量增加，表观解离常数无显著变化，这种变化在肾切除术后15小时变得显著。在切除子宫前立即给肾切除大鼠静脉注射急性升压剂量的血管紧张素II，并不影响子宫血管紧张素受体的数量，而长期输注血管紧张素则会减少受体数量。这些结果解释了在从肾切除大鼠切除的子宫中观察到的对血管紧张素II的特异性超敏反应，这种超敏反应不能用受体位点占据率的变化来解释。这些结果还表明，至少在子宫收缩细胞中，血管紧张素受体的数量受内源性血管紧张素水平慢性变化的影响。
