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1
Employing Escherichia coli to functionally express, purify, and characterize a human transporter.利用大肠杆菌对一种人类转运蛋白进行功能表达、纯化及特性鉴定。
Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8597-601. doi: 10.1073/pnas.132266599. Epub 2002 Jun 19.
2
Site-specific tryptophan fluorescence spectroscopy as a probe of membrane peptide structure and dynamics.
Eur Biophys J. 2002 Mar;31(1):9-13. doi: 10.1007/s002490100182.
3
The E. coli BtuCD structure: a framework for ABC transporter architecture and mechanism.大肠杆菌BtuCD结构：ABC转运蛋白结构与机制的框架
Science. 2002 May 10;296(5570):1091-8. doi: 10.1126/science.1071142.
4
Overproduction in yeast and rapid and efficient purification of the rabbit SERCA1a Ca(2+)-ATPase.酵母中的过量表达以及兔肌浆网Ca(2+) -ATP酶SERCA1a的快速高效纯化。
Biochim Biophys Acta. 2002 Feb 18;1560(1-2):67-83. doi: 10.1016/s0005-2736(01)00458-8.
5
Expression of the human mu opioid receptor in a stable Sf9 cell line.
J Biotechnol. 2002 May 9;95(2):181-7. doi: 10.1016/s0168-1656(02)00008-1.
6
X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity.氯离子通道ClC在3.0埃分辨率下的X射线晶体结构揭示了阴离子选择性的分子基础。
Nature. 2002 Jan 17;415(6869):287-94. doi: 10.1038/415287a.
7
Yeast--a panacea for the structure-function analysis of membrane proteins?酵母——膜蛋白结构-功能分析的万灵药？
Curr Genet. 2001 Oct;40(3):157-71. doi: 10.1007/s002940100252.
8
Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution.钾离子通道与Fab片段复合物在2.0埃分辨率下揭示的离子配位与水合作用化学
Nature. 2001 Nov 1;414(6859):43-8. doi: 10.1038/35102009.
9
Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli.绿色荧光蛋白作为监测大肠杆菌中膜蛋白过表达的指标。
FEBS Lett. 2001 Oct 26;507(2):220-4. doi: 10.1016/s0014-5793(01)02980-5.
10
Structure of MsbA from E. coli: a homolog of the multidrug resistance ATP binding cassette (ABC) transporters.来自大肠杆菌的MsbA的结构：多药耐药ATP结合盒（ABC）转运蛋白的同源物。
Science. 2001 Sep 7;293(5536):1793-800. doi: 10.1126/science.293.5536.1793.

用于哺乳动物膜蛋白结构蛋白质组学的异源表达和纯化系统。

Heterologous expression and purification systems for structural proteomics of mammalian membrane proteins.

作者信息

Mus-Veteau Isabelle

机构信息

Laboratoire de Physiologie Cellulaire et Moléculaire, UMR-CNRS 6548, Université de Nice-Sophia Antipolis, Parc Valrose Nice cedex 2, 06108 France.

出版信息

Comp Funct Genomics. 2002;3(6):511-7. doi: 10.1002/cfg.218.

DOI:10.1002/cfg.218
PMID:18629259
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2448422/
Abstract

Membrane proteins (MPs) are responsible for the interface between the exterior and the interior of the cell. These proteins are implicated in numerous diseases, such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, heart failure, hypertension and Alzheimer's disease. However, studies on these disorders are hampered by a lack of structural information about the proteins involved. Structural analysis requires large quantities of pure and active proteins. The majority of medically and pharmaceutically relevant MPs are present in tissues at very low concentration, which makes heterologous expression in large-scale production-adapted cells a prerequisite for structural studies. Obtaining mammalian MP structural data depends on the development of methods that allow the production of large quantities of MPs. This review focuses on the different heterologous expression systems, and the purification strategies, used to produce large amounts of pure mammalian MPs for structural proteomics.

摘要

膜蛋白（MPs）负责细胞外部与内部之间的界面。这些蛋白质与多种疾病有关，如癌症、囊性纤维化、癫痫、高胰岛素血症、心力衰竭、高血压和阿尔茨海默病。然而，由于缺乏有关所涉及蛋白质的结构信息，对这些疾病的研究受到了阻碍。结构分析需要大量纯净且有活性的蛋白质。大多数与医学和药学相关的膜蛋白在组织中的浓度非常低，这使得在适应大规模生产的细胞中进行异源表达成为结构研究的先决条件。获取哺乳动物膜蛋白结构数据依赖于能够大量生产膜蛋白的方法的开发。本综述重点关注用于为结构蛋白质组学大量生产纯净哺乳动物膜蛋白的不同异源表达系统和纯化策略。