Csejtei Andras, Tibold Antal, Varga Zsuzsa, Koltai Katalin, Ember Agoston, Orsos Zsuzsa, Feher Gergely, Horvath Ors Peter, Ember Istvan, Kiss Istvan
Department of Oncoradiology, Markusovszky Hospital, Markusovszky, Hungary.
Anticancer Res. 2008 May-Jun;28(3B):1917-22.
Cancer of the colorectal region is the second most frequent cause of death among malignant diseases. The influence of two allelic polymorphisms of GSTM1 and GSTT1, and that of p53 gene codon 72 on colon cancer was investigated.
Intraoperatively removed tissue samples were processed from colorectal cancer patients. Cancer-free human samples were used as matched controls. Samples were digested with proteinase-K. DNA solution was used for PCR amplification.
No significant difference was found between tumor patients and controls in the investigated polymorphisms. A significant association was found in Dukes' B stage patients between the GSTM1 and p53 gene variants and survival. In patients with GSTM1 null genotype and p53 Arg/Pro heterozygotes or Pro/Pro homozygotes the chance of survival is significantly lower than in the case of GSTM1+ and p53 Arg/Arg variants (p=0.009 and p=0.008, respectively).
The significance of the investigated polymorphisms in prognosis is dependent on the tumor stage. These parameters might be used in certain cases as prognostic biomarkers in clinical diagnostics and in the planning of individual therapy.
结直肠癌是恶性疾病中第二大常见死因。研究了谷胱甘肽S-转移酶M1（GSTM1）和谷胱甘肽S-转移酶T1（GSTT1）的两个等位基因多态性以及p53基因第72密码子对结肠癌的影响。
从结直肠癌患者术中切除的组织样本进行处理。将无癌的人体样本用作匹配对照。样本用蛋白酶K消化。DNA溶液用于聚合酶链反应（PCR）扩增。
在所研究的多态性中，肿瘤患者与对照之间未发现显著差异。在Dukes' B期患者中，发现GSTM1和p53基因变异与生存率之间存在显著关联。GSTM1基因缺失型和p53 Arg/Pro杂合子或Pro/Pro纯合子患者的生存机会显著低于GSTM1+和p53 Arg/Arg变异患者（分别为p = 0.009和p = 0.008）。
所研究的多态性在预后中的意义取决于肿瘤分期。在某些情况下，这些参数可作为临床诊断和个体化治疗规划中的预后生物标志物。
