Mojtahedi Z, Haghshenas M R, Hosseini S V, Fattahi M J, Ghaderi A
Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran.
Indian J Cancer. 2010 Jan-Mar;47(1):31-4. doi: 10.4103/0019-509X.58856.
The association of a functional single nucleotide polymorphism at codon 72 of the p53 gene (Arg72Pro) with malignancy is a subject of controversy. We analyzed this polymorphism in 224 patients with gastrointestinal cancers (92 with stomach cancer and 132 with colorectal cancer) and in 163 healthy controls.
DNA was extracted from peripheral blood mononuclear cells and amplified with an allele-specific polymerase chain reaction.
There was no significant association between p53 alleles and gastrointestinal cancers. The frequency of the Arg allele was 59.7, 58.8, and 59.2% in the stomach cancer patients, colorectal cancer patients, and controls, respectively. Frequencies of the Pro allele were 40.3% in patients with stomach cancer, 41.2% in patients with colorectal cancer, and 40.8% in controls. Likewise, genotype frequencies did not differ significantly between the two patient groups and controls. There were no differences in genotype or allele frequencies by gender, age, or histological grade.
The data do not support the association of the p53 codon 72 polymorphism with stomach or colorectal cancers in Iranian patients.
p53基因第72密码子处的功能性单核苷酸多态性（Arg72Pro）与恶性肿瘤的关联存在争议。我们分析了224例胃肠道癌症患者（92例胃癌患者和132例结直肠癌患者）以及163例健康对照者的这种多态性。
从外周血单个核细胞中提取DNA，并采用等位基因特异性聚合酶链反应进行扩增。
p53等位基因与胃肠道癌症之间无显著关联。在胃癌患者、结直肠癌患者和对照者中，Arg等位基因的频率分别为59.7%、58.8%和59.2%。胃癌患者中Pro等位基因的频率为40.3%，结直肠癌患者中为41.2%，对照者中为40.8%。同样，两个患者组与对照者之间的基因型频率无显著差异。基因型或等位基因频率在性别、年龄或组织学分级方面无差异。
数据不支持p53第72密码子多态性与伊朗患者的胃癌或结直肠癌有关联。
