Geng Hongran, Zhu Li, Yuan Yuan, Zhang Wei, Li Wenjun, Wang Jie, Zheng Yuling, Wei Kaihua, Cao Wuchun, Wang Hengliang, Jiang Yongqiang
State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, Beijing 100071, People's Republic of China.
J Proteome Res. 2008 Sep;7(9):4132-42. doi: 10.1021/pr800196v. Epub 2008 Jul 16.
Streptococcus suis, a zoonotic pathogen, caused serious outbreaks in humans with high mortality rates in the past decade. To develop safer and more effective vaccines, particularly for human protection, cell wall and extracellular proteins of S. suis serotype 2 were analyzed by an immunoproteomic approach in this study. Thirty-two proteins with high immunogenicity were identified and 22 of them were newly identified. Further analyses of 9 selected proteins revealed that (1) these 9 proteins were expressed in all tested virulent S. suis serotype 2 isolates, (2) antisera against 6 of the selected proteins efficiently killed the bacteria by opsonized phagocytosis in human blood, and (3) significantly higher levels of serum antibodies against 3 proteins were detected in both patients and infected swines. Therefore, our results suggest the 3 proteins (SSU98_0197, SSU98_1094 and SSU1664) have strong potential to be vaccine candidates.
猪链球菌是一种人畜共患病原体，在过去十年中曾导致人类严重疫情爆发，死亡率很高。为了研发更安全、更有效的疫苗，特别是用于人类防护的疫苗，本研究采用免疫蛋白质组学方法分析了猪链球菌2型的细胞壁蛋白和胞外蛋白。鉴定出32种具有高免疫原性的蛋白质，其中22种是新发现的。对9种选定蛋白质的进一步分析表明：（1）这9种蛋白质在所有测试的猪链球菌2型强毒株中均有表达；（2）针对6种选定蛋白质的抗血清通过调理吞噬作用在人血液中有效杀灭细菌；（3）在患者和受感染猪中均检测到针对3种蛋白质的血清抗体水平显著更高。因此，我们的结果表明这3种蛋白质（SSU98_0197、SSU98_1094和SSU1664）具有很强的成为候选疫苗的潜力。
