Zhang Anding, Xie Chunyan, Chen Huanchun, Jin Meilin
Unit of Animal Infectious Diseases, National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Hubei, P. R. China.
Proteomics. 2008 Sep;8(17):3506-15. doi: 10.1002/pmic.200800007.
Streptococcus suis serotype 2 (SS2) is a porcine and human pathogen with adhesive and invasive properties. The absence of suitable vaccine or virulent marker can be the bottleneck to control SS2 infection. An immunoproteome-based approach was developed to identify candidate antigens of SS2 for vaccine development. Hyperimmune sera, convalescent sera, and control sera were analyzed for reactivity by Western Blot against SS2 cell wall-associated proteins (WAPs) separated by 2-DE. A total of 34 proteins were identified by immunoproteomic analysis, of which 15 were recognized by both hyperimmune sera and convalescent sera, including most WAPs currently characterized as SS2 vaccine candidate antigens: muramidase-released protein (MRP), surface protein SP1 (Sao), and glyceraldehyde-3-phosphate dehydrogenase (GapdH). The novel immunogenic proteins may be developed as alternative antigens for further study of SS2 vaccine and diagnostics.
猪链球菌2型（SS2）是一种具有黏附性和侵袭性的猪源及人源病原体。缺乏合适的疫苗或毒力标记可能成为控制SS2感染的瓶颈。为了鉴定用于疫苗开发的SS2候选抗原，开发了一种基于免疫蛋白质组学的方法。通过蛋白质免疫印迹法分析了超免疫血清、恢复期血清和对照血清与经双向电泳分离的SS2细胞壁相关蛋白（WAPs）的反应性。通过免疫蛋白质组学分析共鉴定出34种蛋白质，其中15种被超免疫血清和恢复期血清共同识别，包括目前被鉴定为SS2疫苗候选抗原的大多数WAPs：溶菌酶释放蛋白（MRP）、表面蛋白SP1（Sao）和甘油醛-3-磷酸脱氢酶（GapdH）。这些新的免疫原性蛋白可作为替代抗原，用于SS2疫苗和诊断的进一步研究。
