Krell P J, Stoltz D B
Department of Microbiology, Dalhousie University, Halifax, Nova Scotia, B3H 4H7 Canada.
Virology. 1980 Mar;101(2):408-18. doi: 10.1016/0042-6822(80)90454-7.
Virus-like particles have been isolated from the oviducts of a parasitoid wasp, Hyposoter exiguae (Hymenoptera: Ichneumonidae). Particles are readily purified by centrifugation on either Ficoll or sucrose gradients. Double-stranded circular DNA isolated from purified particles is heterodisperse in terms of molecular weight; none of the molecules are sufficiently large to code for the aggregate of structural proteins comprising the particles. Preliminary Southern blot hybridization data suggest that there is minimal sequence homology between the different size classes of DNA examined.
已从寄生蜂淡足侧沟茧蜂（膜翅目：姬蜂科）的输卵管中分离出病毒样颗粒。通过在菲可或蔗糖梯度上离心可轻松纯化颗粒。从纯化颗粒中分离出的双链环状DNA在分子量方面是异质分散的；没有一个分子足够大到能够编码构成颗粒的结构蛋白聚集体。初步的Southern印迹杂交数据表明，所检测的不同大小类别的DNA之间的序列同源性极小。
