芜菁花叶病毒基因组在芜菁叶分离细胞核中的转录

Transcription of the cauliflower mosaic virus genome in isolated nuclei from turnip leaves.

作者信息

Guilfoyle T J

机构信息

Department of Botany, 220 Bioscience Center, University of Minnesota, St. Paul, Minnesota 55108, USA.

出版信息

Virology. 1980 Nov;107(1):71-80. doi: 10.1016/0042-6822(80)90273-1.

DOI:10.1016/0042-6822(80)90273-1

PMID:18631807

Abstract

Nuclei isolated from turnip (Brassica rapa L. c.v. Just Right) leaves infected with cauliflower mosaic virus synthesize RNA in vitro which hybridizes to purified cauliflower mosaic virus DNA. Nuclei isolated from uninfected leaves do not produce these viral transcripts in vitro. Viral-specific transcription in isolated nuclei is catalyzed by endogenous DNA-dependent RNA polymerase 11 based on sensitivity to alpha-amanitin and ionic strength optima. Only one strand of the viral genome is transcribed in vitro in isolated nuclei. The RNA synthesized in vitro hybridizes to the same strand and EcoRI restriction fragments of cauliflower mosaic virus DNA as the viral-specific RNA that accumulates in vivo in infected turnip leaves.

摘要

从感染花椰菜花叶病毒的芜菁（Brassica rapa L. c.v. Just Right）叶片中分离出的细胞核在体外合成RNA，该RNA能与纯化的花椰菜花叶病毒DNA杂交。从未感染叶片中分离出的细胞核在体外不会产生这些病毒转录本。基于对α-鹅膏蕈碱的敏感性和最佳离子强度，分离细胞核中的病毒特异性转录由内源性DNA依赖性RNA聚合酶II催化。在分离的细胞核中，体外转录的仅为病毒基因组的一条链。体外合成的RNA与花椰菜花叶病毒DNA的同一条链及EcoRI限制性片段杂交，就如同在感染芜菁叶片中体内积累的病毒特异性RNA一样。