Howell S H, Walker L L, Dudley R K
Science. 1980 Jun 13;208(4449):1265-7. doi: 10.1126/science.208.4449.1265.
Cauliflower mosaic virus DNA cloned in the Sal I site of bacterial plasmid pBR322 infects turnip plants. The cloned viral DNA must be excised from the recombinant plasmid to infect, but need not be circularized and ligated in vitro. The cloned viral DNA lacks site-specific single-strand breaks found in DNA obtained directly from the virus. However, these breaks are reintroduced into the viral genome during multiplication of the virus in the plant host.
克隆于细菌质粒pBR322的Sal I位点的花椰菜花叶病毒DNA可感染芜菁植株。克隆的病毒DNA必须从重组质粒中切出才能进行感染，但无需在体外环化和连接。克隆的病毒DNA缺乏直接从病毒获得的DNA中所具有的位点特异性单链断裂。然而，在病毒于植物宿主中增殖期间，这些断裂会重新引入病毒基因组中。
