Laboratoire de Physiologie, Véǵetale Université de Perpignan, Avenue de Villeneuve, F 66025, Perpignan, Cedex, France.
Plant Mol Biol. 1984 Jul;3(4):217-25. doi: 10.1007/BF00029657.
Results obtained in the past few years in the study of the reaction mechanism of plant RNA polymerases are reviewed and discussed. They suggest that valuable information can be obtained using a highly simplified transcription system composed of purified plant enzymes and cloned genes. This type of approach may provide a starting point for the development of an in vitro transcription system. The detailed study of the fundamental enzymatic properties of the plant RNA polymerases allows a comparison with the well documented corresponding bacterial enzyme.
过去几年中,对植物 RNA 聚合酶反应机制的研究结果进行了综述和讨论。这些结果表明,使用由纯化的植物酶和克隆基因组成的高度简化的转录系统可以获得有价值的信息。这种方法可能为体外转录系统的开发提供起点。对植物 RNA 聚合酶基本酶学特性的详细研究,可以与已有充分文献记载的相应细菌酶进行比较。