Santiago A F, Fernandes R M, Santos B P, Assis F A, Oliveira R P, Carvalho C R, Faria A M C
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-901 Belo Horizonte, MG, Brazil.
Cell Immunol. 2008 May-Jun;253(1-2):5-10. doi: 10.1016/j.cellimm.2008.06.004. Epub 2008 Jul 15.
Although it is known that Peyer's patches are the major inductive site for S-IgA production and B1 cells contribute to half of the IgA plasma cells detected in the gut lamina propria, the type of contribution of mesenteric lymph nodes to the process is still unclear. Cytokines such as TGF-beta, IL-10, IL-4, IL-5, and IL-6, are required to promote IgA class switching and IgA synthesis. Aging-related alterations in T and B cells and in cytokine production are already known. Some reports have also proposed that S-IgA production might be altered in aged animals. Herein, we investigated the role of MLN and aging in S-IgA production. Two- to 18-month-old BALB/c mice were used to evaluate aging-related alterations and MLN were removed to study its role in S-IgA production. We found that MLN are important, although not essential for S-IgA production. In addition, we showed that production of IgA-related cytokines are well preserved in MLN but not in PP of aged mice and that S-IgA levels are not affected by aging. Our results suggest that MLN may play a complementary role in S-IgA production mostly in aged animals.
虽然已知派尔集合淋巴结是分泌型免疫球蛋白A（S-IgA）产生的主要诱导部位，且B1细胞占肠道固有层中检测到的IgA浆细胞的一半，但肠系膜淋巴结在这一过程中的作用类型仍不清楚。诸如转化生长因子-β（TGF-β）、白细胞介素-10（IL-10）、白细胞介素-4（IL-4）、白细胞介素-5（IL-5）和白细胞介素-6（IL-6）等细胞因子是促进IgA类别转换和IgA合成所必需的。T细胞和B细胞以及细胞因子产生中与衰老相关的改变已为人所知。一些报告还提出，老年动物的S-IgA产生可能会发生改变。在此，我们研究了肠系膜淋巴结和衰老在S-IgA产生中的作用。使用2至18月龄的BALB/c小鼠来评估与衰老相关的改变，并切除肠系膜淋巴结以研究其在S-IgA产生中的作用。我们发现肠系膜淋巴结很重要，尽管对于S-IgA的产生并非必不可少。此外，我们表明，老年小鼠的肠系膜淋巴结中与IgA相关的细胞因子产生保存良好，但派尔集合淋巴结中则不然，并且S-IgA水平不受衰老影响。我们的结果表明，肠系膜淋巴结可能在S-IgA产生中发挥互补作用，主要是在老年动物中。
