Department of Molecular Bioscience, School of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.
Immune Netw. 2009 Aug;9(4):133-7. doi: 10.4110/in.2009.9.4.133. Epub 2009 Aug 31.
We have recently shown that activin A, a member of TGF-beta superfamily, stimulates mouse B cells to express IgA isotype but other isotypes. In the present study, we further characterized effects of activin A on B cell growth and IgA expression. We found that activin A did not have effect on LPS-stimulated cell viability. In parallel, CFSE staining analysis revealed that activin A did not alter cell division. An increase of IgA secretion by activin A was completely abrogated by anti-activin A Ab but not by anti-TGFbeta1 Ab. In the same conditions, no other isotypes are significantly affected by each antibody treatment. Finally, activin A, as similar to TGF-beta1, increased IgA secretion by mesenteric lymph node cells. These results suggest that activin A can specifically stimulate IgA response, independent of TGF-beta in the gut.
我们最近发现，属于 TGF-β超家族的激活素 A 可刺激小鼠 B 细胞表达 IgA 同种型，但不表达其他同种型。在本研究中，我们进一步研究了激活素 A 对 B 细胞生长和 IgA 表达的影响。我们发现激活素 A 对 LPS 刺激的细胞活力没有影响。同时，CFSE 染色分析表明激活素 A 不会改变细胞分裂。抗激活素 A Ab 完全阻断了激活素 A 引起的 IgA 分泌增加，但抗 TGF-β1 Ab 则没有。在相同条件下，每种抗体处理均不会显著影响其他同种型的分泌。最后，激活素 A 与 TGF-β1 相似，可增加肠系膜淋巴结细胞的 IgA 分泌。这些结果表明，激活素 A 可特异性刺激肠道中的 IgA 反应，而不依赖于 TGF-β。
