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以无脂肪族13Cγ的氨基酸残基的C末端邻位识别辅助蛋白质的核磁共振谱峰归属

Identification of C-terminal neighbours of amino acid residues without an aliphatic 13Cgamma as an aid to NMR assignments in proteins.

作者信息

Barnwal Ravi Pratap, Rout Ashok K, Atreya Hanudatta S, Chary Kandala V R

机构信息

Department of Chemical Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, India.

出版信息

J Biomol NMR. 2008 Aug;41(4):191-7. doi: 10.1007/s10858-008-9254-2. Epub 2008 Jul 17.

DOI:10.1007/s10858-008-9254-2
PMID:18633715
Abstract

We propose a methodology that uses GFT (3,2)D CB(CACO)NNH experiment to rapidly collect the data and readily identify six amino acid residue types (Ala, Asn, Asp, Cys, Gly and Ser) in any given protein. Further, the experiment can distinguish the redox state of Cys residues. The proposed experiment in its two forms will have wide range of applications in resonance assignment strategies and structure determination of proteins.

摘要

我们提出了一种方法，该方法使用GFT(3,2)D CB(CACO)NNH实验来快速收集数据，并能够轻松识别任何给定蛋白质中的六种氨基酸残基类型（丙氨酸、天冬酰胺、天冬氨酸、半胱氨酸、甘氨酸和丝氨酸）。此外，该实验可以区分半胱氨酸残基的氧化还原状态。所提出的两种形式的实验将在蛋白质的共振归属策略和结构测定中具有广泛的应用。

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