Stasiak-Barmuta Anna, Stankiewicz Wanda, Chciałowski Andrzej, Moniuszko Tadeusz, Alifier Marek
Samodzielny Publiczny Dzieciecy Szpital Kliniczny w Białymstoku, Pracownia Cytometrii Przepływowej.
Pol Merkur Lekarski. 2008 Jan;24(139):8-13.
THE AIM OF THIS STUDY was the evaluation of T lymphocytes and NK function in patients with non-allergic asthma.
Three groups: patients with non-allergic asthma (n = 30), patients with non-allergic asthma with concomitant recurrent infections (n = 24) and group of healthy adult volunteers (n = 20) were examined. CD4+ and NK cells were isolated from peripheral blood by magnetic separation method. Cells were cultured and level of cytokine IL-10, IL-13, IFN-gamma in medium were measured.
In patients with non-allergic asthma with recurrent infections mean value of IFN-gamma in medium from IL-15 + IL-12 stimulated NK cells was significantly lower compared with control group (p < 0.01). Mean value of IL-10 and IL-13 in medium from Con A stimulated CD4+ cells in patients with non-allergic asthma with recurrent infections were significantly higher than in control group (p < 0.05).
Multitudes of cell and humoral factors interactions overlap dysfunction of immunological system in patients with non-allergic asthma. It causes higher susceptibility to infections factors especially viruses. Viruses give feedback for escalation of infection and worsening of clinical course of disease. Knowing mechanisms of inflammation intensification would let us specific prophylaxis use (specific antinflammatory or immunomodulatory drugs).
本研究的目的是评估非过敏性哮喘患者的T淋巴细胞和自然杀伤细胞（NK）功能。
研究对象分为三组：非过敏性哮喘患者（n = 30）、伴有反复感染的非过敏性哮喘患者（n = 24）和健康成年志愿者组（n = 20）。通过磁性分离法从外周血中分离出CD4 +细胞和NK细胞。对细胞进行培养，并测定培养基中细胞因子白细胞介素-10（IL-10）、白细胞介素-13（IL-13）、干扰素-γ（IFN-γ）的水平。
在伴有反复感染的非过敏性哮喘患者中，IL-15 + IL-12刺激的NK细胞培养基中IFN-γ的平均值显著低于对照组（p < 0.01）。伴有反复感染的非过敏性哮喘患者中，刀豆蛋白A（Con A）刺激的CD4 +细胞培养基中IL-10和IL-13的平均值显著高于对照组（p < 0.05）。
多种细胞和体液因子相互作用导致非过敏性哮喘患者免疫系统功能失调。这使得患者对感染因素尤其是病毒更易感。病毒会反馈导致感染升级和疾病临床病程恶化。了解炎症加剧的机制将有助于我们进行特异性预防（使用特异性抗炎或免疫调节药物）。
