Tavakkol Afshari Jalil, Farid Hosseini Reza, Hosseini Farahabadi Sara, Heydarian Farhad, Boskabady Mohammad Hossein, Khoshnavaz Rasoul, Razavi Alireza, Ghayoor Karimiani Ehsan, Ghasemi Gholamreza
Department of Immunogenetics, Immunology Research Centre, Bu-Ali Research Institute, Mashhad, Iran.
Iran J Allergy Asthma Immunol. 2007 Jun;6(2):67-72.
Immune and inflammatory responses mediated by cytokines, play important roles in the pathophysiology of asthma. These responses are associated with overexpression of Th2 cytokines such as IL-4 and IL-13. These two cytokines use common receptors for signaling that lead to identical immunological effects and regulation of the Th1/Th2 balance. The aim of this study was to determine whether patients with allergic asthma display overexpression of IL-4 and IL-13 genes. Using RT-PCR, we examined the expression of IL-4 and IL-13 genes in twenty asthmatic cases and twenty normal individuals. Total levels of serum IgE and IL-4 were also determined by ELISA method. Expression of IL-13 gene in 70% of patients with allergic asthma was higher than controls (P=0.01). There was no correlation between the expression of IL-13 gene and total level of serum IgE (P=0.07). Expression of IL-4 gene was detected in 30% of the patients and none of the normal individuals as determined by RT-PCR (P=0.01). Mean of serum IgE levels in patients and controls were 84.9 IU/ml and 62.2 IU/ml, respectively. Level of serum IgE was more than 100 IU/ml in 30% of patients (P=0.03). Mean of serum IL-4 levels in patients and controls were 15.73 pg/ml and 13.07 pg/ml, respectively. There was a relation between levels of serum IgE and IL-4 in 73% of cases. The results showed that there was a correlation between the expression of IL-4 gene and the level of serum IL-4. Levels of serum IgE and IL-4 were considerably higher in asthmatics than non-asthmatic controls.
由细胞因子介导的免疫和炎症反应在哮喘的病理生理学中起重要作用。这些反应与Th2细胞因子如白细胞介素-4（IL-4）和白细胞介素-13（IL-13）的过度表达有关。这两种细胞因子使用共同的受体进行信号传导，从而导致相同的免疫效应并调节Th1/Th2平衡。本研究的目的是确定过敏性哮喘患者是否表现出IL-4和IL-13基因的过度表达。我们使用逆转录聚合酶链反应（RT-PCR）检测了20例哮喘患者和20例正常个体中IL-4和IL-13基因的表达。血清总免疫球蛋白E（IgE）和IL-4水平也通过酶联免疫吸附测定（ELISA）方法进行了测定。70%的过敏性哮喘患者中IL-13基因的表达高于对照组（P = 0.01）。IL-13基因的表达与血清总IgE水平之间无相关性（P = 0.07）。通过RT-PCR检测，30%的患者中检测到IL-4基因的表达，而正常个体中均未检测到（P = 0.01）。患者和对照组的血清IgE平均水平分别为84.9国际单位/毫升和62.2国际单位/毫升。30%的患者血清IgE水平超过100国际单位/毫升（P = 0.03）。患者和对照组的血清IL-4平均水平分别为15.73皮克/毫升和13.07皮克/毫升。73%的病例中血清IgE和IL-4水平之间存在关联。结果表明，IL-4基因的表达与血清IL-4水平之间存在相关性。哮喘患者的血清IgE和IL-4水平明显高于非哮喘对照组。
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