Gould A R
Department of Plant Pathology, Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, 5064, South Australia.
Virology. 1981 Jan 15;108(1):123-33. doi: 10.1016/0042-6822(81)90532-8.
A viroid-like RNA has been purified from velvet tobacco mottle virus (VTMoV) capsids. This RNA exists as a covalently closed circular molecule of RNA as well as in a linear form with a molecular weight of approximately 1.2 x 10(5). Complementary DNA (cDNA) analysis indicates that the circular and linear forms of the viroid-like RNA contain indistinguishable nucleotide sequences and are unique RNA species. The viroid-like RNA has no nucleotide sequence homology with any other encapsidated RNAs and exhibits an absorbance-temperature profile, as well as nuclease sensitivities, typical of a viroid. The three other RNAs encapsidated in VTMoV virions were also investigated by cDNA analysis. Two of these (designated RNAs 1a and 1b, with molecular weights of 0.63 x 10(6) and 0.25 x 10(6), respectively) are shown to be breakdown fragments of the largest RNA encapsidated by VTMoV (designated RNA 1) with a molecular weight of 1.5 x 10(6).
一种类病毒样RNA已从绒毛烟草斑驳病毒（VTMoV）衣壳中纯化出来。这种RNA以共价闭合环状RNA分子形式存在，也有线形形式，分子量约为1.2×10⁵。互补DNA（cDNA）分析表明，类病毒样RNA的环状和线性形式含有难以区分的核苷酸序列，是独特的RNA种类。该类病毒样RNA与任何其他被衣壳包裹的RNA均无核苷酸序列同源性，并且呈现出类病毒典型的吸光度-温度曲线以及核酸酶敏感性。对VTMoV病毒粒子中包裹的其他三种RNA也进行了cDNA分析。其中两种（分别命名为RNA 1a和1b，分子量分别为0.63×10⁶和0.25×10⁶）被证明是VTMoV包裹的最大RNA（命名为RNA 1，分子量为1.5×10⁶）的降解片段。
